Table 1.
Strains used in this study.
Figure 1.
The engineered taxadiene biosynthetic pathway in S. cerevisiae.
Figure 2.
Production of taxadiene by engineered S. cerevisiae strains SyBE_001188, SyBE_001189, SyBE_001190 and SyBE_001110.
Data are represented as mean value of production from three independent fermentations.
Figure 3.
Docking result of FPP with (A) GGPPSbc; (B) GGPPSgb; (C) GGPPSrc; (D) GGPPSeh; (E) GGPPScr; (F) GGPPSsc.
In A-F, the left figure represent the whole protein-substrate docking; the right figure is site gain.
Table 2.
Data of enzyme-substrate docking.
Figure 4.
Production of taxadiene by engineered S. cerevisiae strains SyBE_001109, SyBE_001110, SyBE_001111, SyBE_001113, SyBE_001114, and SyBE_001115.
Data are represented as mean value of production from three independent fermentations.
Figure 5.
The contents of identified metabolites and amino acids in glycolytic pathway and TCA cycle of W303-1A and YSG50.
The y-axis was relative abundance which was calculated by normalization of peak area of each metabolite to internal standard, and each value represented mean value of two independent replicates.
Figure 6.
The relative abundance of TCA cycle intermediates (citrate, succinic acid and fumaric acid) in taxadiene producing yeast SyBE_001113, SyBE_001114, and SyBE_001115 compared with those in YSG50 chassis.