Figure 1.
Evaluation of As+3 inhibition of HPBMC proliferation.
In panel A, the typical response of 15 donors is shown where there is no inhibition of PHA-induced T cell proliferation. In panel B, 2 donors are shown that were extremely sensitive to low dose (0.1–1 nM) inhibition of the PHA response. Data shown are the Mean ± SD with *p<.05.
Table 1.
Arsenite Suppresses Anti-CD3/Anti-CD28 Naïve HPBMC T cell Differentiation.
Figure 2.
Correlation analysis of MMA in urine with PHA-induced T cell stimulation (3H-Thymidine counts per minute (CPM).
There is an indication of a weak correlation between urinary MMA (combined MMA+3 and MMA+5) amounts and CPM in the 13 donors analyzed. Values are log transformed. R = −0.46, p = 0.24.
Table 2.
PHA-Induced 3H-Thymidine Stimulation and Urinary Arsenic (Inorganic + Organic) in Normal Human Blood Donors.
Figure 3.
Proliferation response of PHA and anti-CD3/anti-CD28 stimulated PBMC treated with As+3.
Comparison of PHA stimulated (A) and anti-CD3/anti-CD28 stimulated (B) T cell proliferation (3H-thymidine) for potential As+3 inhibition in 17 donors. Data shown are the Mean (red line) and Median (black line) ± SD with *p<.05.
Figure 4.
As+3 treated compared to MMA+3 treated PBMC stimulated with either PHA or anti-CD3/anti-CD28.
Comparison of As+3 inhibition for PHA (A) and anti-CD3/anti-CD8 (C) and MMA+3 inhibition of T cell proliferation induced by PHA (B) and anti-CD3/anti-CD28 (D) in 8 donors. Data shown are the Mean (red line) and Median (black line) ± SD with *p<.05.