Figure 1.
Representative 2-DE serum protein profiles of normal controls and OSCC patients.
Unfractionated serum samples of (a) normal controls and (b) OSCC patients were subjected to 2-DE and silver staining. The labeled spot clusters are proteins which are consistently identified in profiles of normal controls and OSCC patients. α2-HS-glycoprotein (AHS) and α1-antitrysin (AAT) are high abundance proteins that typically appeared in protein profiles.
Table 1.
The relative expression of host specific proteins among the sera of patients.
Table 2.
Mass spectrometric identification of host-specific protein spots from serum protein profiles using MASCOT search engine and the NCBI database.
Table 3.
Mass spectrometric identification of non-host specific protein spots from serum protein profiles using MASCOT search engine and the NCBI database.
Table 4.
Host- and non-host specific proteins on the 2-DE immunoblots.
Figure 2.
Results from 2-DE immunoblots for (a) normal pooled sera probed with normal pooled sera, (b) normal pooled sera probed with OSCC pooled sera, (c) OSCC pooled sera probed with normal pooled sera, (d) OSCC pooled sera probed with OSCC pooled sera.
Unfractionated, pooled serum samples from control and OSCC patients were subjected to 2-DE and blotted onto nitrocellulose membranes, which were then probed with pooled sera and monoclonal anti-human IgM-HRP.
Table 5.
Functional annotation analysis of identified host-specific proteins using DAVID v6.7.
Figure 3.
Interaction networks of identified host specific proteins using STRING v9.1.
STRING database is a curated knowledge database of known and predicted protein-protein interactions. Most of the identified host-specific proteins have an established link with each other in the interaction network.