Table 1.
Clinico-pathological characteristics of patients cohort.
Table 2.
miRNAs differentially expressed among TCGA subclasses sorted by the p-value of the univariable test.
Figure 1.
miRNA expression analysis of gliomas.
A) Global views of gene expression by using the Principal Component Analysis (PCA) between grade III+IV tumours (HGG, red), and grade II gliomas (LGG, blue) samples and normal brain tissues (NBT, green). The analysis was performed by Partek Genomic Suite software using default setting that includes a threshold to remove low background level intensities. PCA percent mapping on the top of the plot indicates the explained variability on the first coordinates. B) Venn diagrams summarizing the number of miRNAs found to be differentially expressed by comparing grade II gliomas and NBT (green), grade II and grade III+IV gliomas (blue), grade III+IV gliomas and NBT (red). Also, number for conjoint (and non-conjoint) differentially expressed miRNAs are indicated. C) Hierarchical clustering analysis of miRNA expression profile for 8 grade II (blue) and 24 grade III+IV gliomas (red). Columns represent tissue specimens and rows miRNAs.
Figure 2.
Validation of microarray data by qRT PCR analysis.
The 32 glioma samples and 4 NBT were analyzed by RT-qPCR for the expression of the 22 miRNAs identified by microarray profiling. Boxplots of miRNAs expression in normal brain tissues (NBT), WHO grade II gliomas (II) and WHO grade III and IV gliomas (III+IV). Expression levels are expressed as: target miRNA/RNU48 multiplied by 1000. The boxes mark the interquartile range, (interval between the 25th and 75th percentile). The lines inside the boxes denote median values, the symbol * denote the outliers. #NBT vs II vs III+IV comparison by Kruskall Wallis Test; §II vs III+IV comparison by Mann Whitney test.
Figure 3.
Relationship between miRNAs expression and survival in glioma patients.
A) Kaplan-Meier curves showing the relationship of miR-155, miR-21, miR-210 and miR-22 with Overall Survival in patients from the GBM dataset. B) Kaplan- Meier curves showing the relationship of miR-155, miR-1296, and miR-383 with Overall Survival in Grade III glioma patients from the LGG dataset.