Table 1.
Strains used in this study.
Table 2.
PCR primers used in this study.
Figure 1.
PCR amplifications of 16S rRNA with eubacterial and myxobacteria-specific primers.
A. Primer 27F/1492R; B, D and E. Primer mglA1F/mglA1R; C, F and G. Primer mglA2F/mglA1R.
Figure 2.
DGGE analyses of mglA gene fragments performed using DNA samples extracted from pure myxobacteria cultures (A) and soil samples (B).
A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.
Table 3.
Sequence similarity of excised DNA fragments.
Figure 3.
Phylogenetic tree showing the relationship of the twenty-eight retrieved bands from DGGE profile, on the basis of mglA gene fragment sequences obtained.
The numbers of the sequences in this tree (e.g., B1) refer to the numbers in the denaturing gradient gel (i.e., 1 [Fig. 2B]). The tree was constructed using the neighbor-joining method and analysis was based on 194 nucleotides. Numerals at nodes indicate bootstrap values derived from 1000 replications [29].