Figure 1.
The morphology of two tested P. marneffei strains after 7 days of incubation at 25°C and 37°C (a).
Survival curves of C. elegans after infected with P. marneffei in solid medium (b) and liquid medium (c).
Figure 2.
Progression of red pigment in the C. elegans intestine.
Microscopy of wild-type C. elegans N2 at different time points after infected with P. marneffei strain 570 or 486. Red pigment was seen in the C. elegans swelling intestine and increased gradually with time after infected with live P. marneffei strain 570 (a). No obvious swelling or red pigment was seen in the C. elegans intestine after infected with live P. marneffei strain 486 (b).
Figure 3.
P.marneffei hyphae disrupt the nematode cuticle and internal structures.
C. elegans were pre-incubated with conidia of P. marneffei for 4 hours and transferred to pathogen-free medium. Hyphae protruded from the nematode cuticle after infected with P. marneffei strain 570 (a, b) or strain 486 (d). Percent hyphal killing of C. elegans by different P. marneffei strains (c). Images and data were taken 96 hours post infection.
Figure 4.
Antifungal agents prolong the survival of wild-type C. elegans N2 infected by P. marneffei 570.
Significant differences were found between 570 infected group and all the administration groups (P<0.001).