Table 1.
Exserohilum and Bipolaris ITS sequences used for phylogenetic analysis.
Figure 1.
Conidia of (A) clinical isolate of E. rostratum, (B) Bipolaris sp. (C) E. rostratum from plant (P1) with hilum (marked by arrow) viewed under the compound microscope (bars = 10 µm).
Figure 2.
Maximum likelihood phylogeny of the Exserohilum and Bipolaris isolates relative to other E. rostratum clinical and plant isolates and Exserohilum species.
Isolates used in this study are shown in bold. E. rostratum isolates from plants are shown in green and clinical isolates are shown in red. Bootstrap values are shown for branches with values greater than 50%. Branch lengths are in substitutions per site.
Figure 3.
Exserohilum leaf spot (marked by arrow) on (A) stilt grass, (B) bahia grass and (C) rye grass after inoculation with the clinical isolate of Exserohilum rostratum at six days after inoculation.
Figure 4.
Effect of Exserohilum and Bipolaris isolates (A) and plant species (B) on % infection at six days after inoculation.
Data are the means ± standard error of the second trial of the first experiment of the study, four replications (three pots per replication per treatment). There were no significant differences between the isolates of Exserohilum or Bipolaris at P<0.05. Plant species differed significantly in disease intensity (%) in reaction to isolates of Exserohilum or Bipolaris. Different letters above the bar indicate significant differences among plant species (P = 0.0003, Tukey test).
Table 2.
Number of pots with Exserohilum leaf spots out of the total number of pots with plants inoculated with strains of Exserohilum or Bipolaris at six days after inoculation.
Figure 5.
Reaction of stilt grass and rye grass to a clinical (ER C) and plant (ER P1) isolate of Exserohilum rostratum expressed as % infection over time (A) and area under disease progress curve (B) in the second experiment.
Prior to regression analysis, diseased area data were log-transformed, but nontransformed means are presented in the figure for uniformity of presentations. Data are the means ± SE of two repetitions of the study, each with four replications (four per rep per treatment, five plants per pot). Significant differences are marked with different letters above bars (P≤0.05, Tukey-test).