Figure 1.
Deletion of Saa3 attenuates weight gain on a HFHSC diet.
(A) Male and (B) female Saa3+/+ and Saa3−/− mice were fed chow or a high fat, high sucrose + cholesterol (HFHSC) diet for 16 weeks, and weekly body weights recorded. (C–D) Gonadal white adipose tissue (gWAT) and (E–F) liver weight were recorded at sacrifice and normalized to total body weight. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; KO: Saa3−/−.
Figure 2.
Plasma triglycerides, cholesterol, and lipoprotein profiles are improved in female Saa3−/− mice.
(A–B) Triglycerides and (C–D) total cholesterol were measured from fasted plasma after 16 weeks on chow or HFHSC diet. (E–F) Lipoprotein profiles were obtained by fast-phase liquid chromatography (FPLC) of pooled fasted plasma samples taken at sacrifice. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; KO: Saa3−/−.
Figure 3.
Gonadal white adipose tissue inflammatory and chemotactic gene expression is attenuated in female Saa3−/− mice.
(A–H) Total RNA from whole gonadal white adipose tissue (gWAT) was reverse transcribed into cDNA for quantitative PCR analysis. Genes including Saa3 (A–B), Saa1 (C–D), Tnf (E–F), and Ccl2 (G–H) are presented, normalized to an internal control gene (Gapdh) and presented as fold change from Saa3+/+ chow controls. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; HET: Saa3+/−; KO: Saa3−/−.
Table 1.
Relative expression of Saa subtypes in gWAT.
Figure 4.
Macrophage content of gonadal white adipose tissue is decreased in female Saa3−/− mice.
(A–D) Total RNA from whole gonadal white adipose tissue (gWAT) was reverse transcribed into cDNA for quantitative PCR analysis. Genes including Mac2 (A–B) and Emr1 (C–D) are presented, normalized to an internal control gene (Gapdh) and presented as fold change from Saa3+/+ chow controls. (E–G) gWAT was fixed in formalin and embedded in formalin before sectioning and staining with a Mac2 antibody. (E) Representative images are shown, 10X magnification. (F–G) Quantification of the percentage of total Mac2-stained area in all tissue sections examined. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; KO: Saa3−/−.
Figure 5.
Liver Saa1 and Saa2 are attenuated in Saa3−/− mice.
Total RNA from whole liver was reverse transcribed into cDNA for quantitative PCR analysis. Genes including Saa1 (A–B), Saa2 (C–D), Saa3 (E–F), Mac2 (G–H), Emr1 (I–J), and Ccl2 (K–L) are presented, normalized to an internal control gene (Gapdh) and presented as fold change from Saa3+/+ chow controls. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; KO: Saa3−/−.
Figure 6.
Plasma SAA is decreased in both male and female Saa3−/− mice.
(A–B) Total SAA was measured from fasted plasma taken at sacrifice by ELISA. n = 6–15 mice per group. *P<0.05 from chow group; #P<0.05 from Saa3+/+ controls. WT: Saa3+/+; KO: Saa3−/−.
Table 2.
Relative expression of Saa subtypes in liver.