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Figure 1.

Soil microbial biomass C and soil microbial biomass N in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer. Data are means ± SD (n = 3). Different lower case letters indicate significant differences (ANOVA, LSD test, p<0.05) among fertilizer treatments; different upper case letters indicate significant differences among maize growth stages.

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Figure 2.

Soil respirations rate in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer. Data are means ± SD (n = 3). Different lower case letters indicate significant differences (ANOVA, LSD test, p<0.05) among fertilizer treatments; different upper case letters indicate significant differences among maize growth stages.

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Figure 3.

The clony-forming units of bacteria, fungi and actinomyces in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer. Data are means ± SD (n = 3). Different lower case letters indicate significant differences (ANOVA, LSD test, p<0.05) among fertilizer treatments; different upper case letters indicate significant differences among maize growth stages.

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Figure 4.

DGGE banding patterns of 16S bacteria fragment and clustering of DGGE profiles in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer.

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Table 1.

Effect of different treatments on soil bacterial community structure diversity as evaluated by Shannon index (H), Evenness index (E) and Simpson index (C) in the DGGE-PCR.

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Figure 5.

DGGE banding patterns of 18S rDNA fungi fragment and clustering of DGGE profiles in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer.

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Table 2.

Effect of different treatments on soil fungi community structure diversity as evaluated by Shannon index (H), Evenness index (E) and Simpson index (C) in the DGGE-PCR.

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Table 2 Expand

Figure 6.

Soil enzyme activity in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer. Data are means ± SD (n = 3). Different lower case letters indicate significant differences (ANOVA, LSD test, p<0.05) among fertilizer treatments; different upper case letters indicate significant differences among maize growth stages.

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Table 3.

Correlation of MBC, MBN, Available N, SOM, Humus and enzymes activities.

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Table 3 Expand

Figure 7.

PCA of indexes combined with a hierarchical clustering analysis in various treatments during maize growth stages.

CK: no fertilizer; N: N fertilizer; B: bacteria fertilizer; NB: N fertilizer + bacteria fertilizer; M: manure compost; MB: manure compost + bacteria fertilizer. A: Seeding stage; B: Tasseling stage; C: Mature stage.

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