Figure 1.
Schematic representation of the data analysis procedure in the spmratIHEP.
The procedures of spatial normalization showed in the red dashed pane could be accomplished automatically in this toolbox.
Figure 2.
The constructed rat brain template.
(A) Axial, sagittal and coronal views of the standard FDG-PET template with extracranial tissues in Paxinos space; (B) axial, sagittal and coronal views of the corresponding FDG-PET canonical brain; and (C) axial, sagittal and coronal views of the corresponding intracranial mask image in Paxinos space. The cross point of red lines represent the origin point D3V. The origin point was the same in the images of template, canonical brain and intracranial mask.
Figure 3.
Panel A shows three planes from the standard FDG-PET template of rat brain, whose coordinates were Zbregma 2.04 mm, Zbregma −2.28 mm and Zbregma −8.28 mm separately. Panel B shows the extraction result of a rat which is randomly selected from dataset obtained for template construction. Panel C and Panel D show the extraction results of two rats which are randomly selected for dataset obtained for the intracranial brain extraction evaluations. The original image of individual is shown on the left of Panels B, C, D separately. The extracted intracranial tissue of individual is shown on the right of Panels B, C, D separately, of which the left half shows the intracranial mask image superimposing on the extracted canonical brain and the right half shows the extracted canonical brain. The intracranial mask image is presented as a binary image with 25% transparency, while the extracted canonical brain is presented as a background.
Table 1.
Volumetric and spatial correspondence measures between manually traced out intracranial tissues from three experts, of which the result is shown as ‘the mean value ± standard deviation’.
Table 2.
Volumetric and spatial correspondence measures between three manually traced out and automatically extracted intracranial tissues, of which the result is shown as ‘the mean value ± standard deviation’.
Figure 4.
The constructed synthetic FDG-PET images from atlas images in Paxinos & Watson space.
It was shown in pseudo-color scaled and the color-bar stands for the intensity of each voxel in synthetic FDG-PET image. The six main anatomy structures were labeled, in which (1) stands for the olfactory bulb, (2) stands for the cortex, (3) stands for the hippocampi, (4) stands for the mesencephalon, (5) stands for the thalamus and (6) stands for the cerebellar.
Figure 5.
Superimposing the co-registered FDG-PET canonical brain on the MRI T2WI structural canonical brain in Paxinos & Watson space.
The co-registered FDG-PET canonical brain is presented with translucency and pseudo-color scaled. The MRI T2WI canonical brain is presented in gray-scale as a background. The color-bar stands for the intensity of each voxel in FDG-PET canonical brain, which is not translucent.
Figure 6.
The result of two-sample t-test between the MCAO and healthy controls.
(A) The projection of all the blobs were shown in a figure of rat brain, in which the red vees point to the global maximal t-value. (B) The display of the statistical result overlaid on axial, sagittal and coronal views of a structural single brain in Paxinos & Watson space, which is the three-dimensional illustration of one blob. And the color bar stands for the t-value of each significant voxel in Paxinos & Watson space.
Table 3.
The statistical result of two sample t-test between the MCAO and healthy controls.