Table 1.
Strains, plasmids and primers used in this study.
Figure 1.
Site-directed mutagenesis of the lysine residues of the dndE gene in pJTU1238.
The indicated mutations of key lysine residues (K17, K18, K20, K53, K87 and K91) were constructed in pJTU1238, a plasmid harboring the dndBCDE genes of S. enterica. The asterisks denote the selected lysine residues that were replaced with alanines. The residues marked in red indicate the amino acids that are deleted in the dndE in-frame deletion mutant.
Table 2.
PT modifications in E. coli DH10B harboring pJTU1238 and its derivatives.
Table 3.
Total PT modifications normalized to dndE transcript levels.
Figure 2.
The impact of the mutagenesis of the lysine residues on the total PT modifications.
A. The plasmids pWHU668-pWHU674 possessing mutated lysine residues were expressed in E. coli DH10B and XTG102, and the DNA PT frequencies were measured by LC-MS/MS. The columns and error bars represent the mean ± SD; n = 3, biological triplicates. B. qRT-PCR expression analysis of the dndE genes in E. coli DH10B (white bars) and XTG102 (grey bars). The expression levels were normalized to the transcript levels of GAPDH. The columns and error bars represent the mean ± SD; n = 3, biological triplicates. C. PT modifications were normalized to the average dndE expression levels.
Table 4.
PT modifications in XTG102 harboring pJTU1238 and its derivatives.