Figure 1.
Effect of 10 µM resveratrol on NIS gene and β-actin RNA levels.
FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol. A, Effects of 10 µM resveratrol after 48 h of treatment. Insert: Representative Northern blot. B, Effects of resveratrol 10 µM as a function of time. Data are the normalized means ±SD (against β-actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 µM resveratrol. *, p<0.05 versus relevant control.
Figure 2.
Effects of 10 µM resveratrol on NIS protein expression.
FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol for 48 h. A representative Western blot is shown (top), with quantification (bottom). Data are the normalized means ±SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 µM resveratrol. *, p<0.05 versus relevant control.
Figure 3.
Effects of 10 µM resveratrol on NIS protein expression as a function of time.
FRTL-5 cells were grown in 6H5% medium (containing TSH 1 mU/ml) and treated for the indicated time with 10 µM resveratrol. Data for quantification following Western blotting are the normalized means ±SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 µM resveratrol. * p<0.05 versus relevant controls.
Figure 4.
Effects of resveratrol on TSH induction of iodide uptake.
FRTL-5 cells were grown in 12-well plates to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days. TSH (1 mU/ml) was then added without and with resveratrol. A, Effects of resveratrol as a function of concentration after 48 h of treatment. B, Effect of resveratrol as a function of time. Data (from pmole per µg DNA) are normalized means ±SD from three independent experiments (control vehicle, 100%). Control, cells maintained in 5H5% medium (without TSH); TSH, cells with 1 mU/ml TSH + control vehicle (0.5% ethanol); Resv 10, 20, 40, cells with TSH 1 mU/ml + 10, 20, 40 µM resveratrol, respectively. * p<0.05 versus relevant control.
Figure 5.
Effects of resveratrol on radioiodine uptake by the thyroid gland in vivo.
Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 6) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 6), for 14 days. On the last day of treatment, the animals received [125I]-NaI (185 kBq i.p., each) 24 h prior to sacrifice. Their thyroids were removed and weighed, with their associated radioactivity (RAIU) determined in a gamma counter. Data (from iodide uptake per thyroid weight) are normalized means ±SD (control vehicle, 100%). *, p<0.05.
Figure 6.
Effects of resveratrol on NIS protein expression in rat thyroid glands.
Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15th, the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.