Figure 1.
Length distribution of transcripts obtained from O. vulgaris hemocytes transcriptome library.
Figure 2.
Frequency of contigs showing similarity to known proteins in the NCBI database.
Table 1.
Summary statistics of sequencing and assembly for O. vulgaris hemocytes transcriptome.
Figure 3.
Top 35 hit sequences matching O. vulgaris assembled sequences.
Figure 4.
Distribution of second level GO annotation in three categories: (A) cellular component, (B) molecular function and (C) biological process.
Figure 5.
Complement and clotting pathway.
Brown figures indicate proteins identified in the O. vulgaris library; and blue figures indicate the absent ones. C1q: Complement C1q binding protein; C1R: C1r subcomponent; C1S: Complement C1 subcomponent; C2: Complement component 2; C4: Complement component 4; C3: Complement component 3; C3a: anaphylatoxin subcomponent 3a; C3b: Opsonin subcomponent 3b; C5: Complement component; C3R: C3 receptor; C5R: C5 receptor; MBL: Manose-binding lectin; MASP1/2: Mannan-binding lectin serine protease 1/2; F12: factor 12; F11: Factor 11; α2M: Alpha-macroglobulin; F2,3,5,7,8,9,10: Coagulation factors 2,3,5,7,8,9,10; MPC: CD46, membrane cofactor protein; VWF: Von Willebrand factor; KLKB1: kallikrein B1; PROC: protein C; PROS1: protein S (alpha); THBD: trombomodulin.
Figure 6.
Brown rectangles indicate proteins identified in the present Octopus vulgaris library and blue rectangles indicate the absent ones. Brown rectangles with red letters indicate proteins in the NF-κB pathway. AKT: RAC-alpha serine/threonine-protein kinase; API1: Transcription factor AP-1; Casp8: Caspase 8; FADD: FAS-associated via death domain; IκB: Inhibitor of NF-κB; IKKε: Inhibitor of nuclear factor kappa-B kinase subunit epsilon; IRAK4: Interleukin-1 receptor-associated kinase 4; IRF3: Interferon regulatory factor 3; IκBα: NF-kappa-B inhibitor alpha; JNK: c-Jun N-terminal kinase; MEKK1: Mitogen-activated protein kinase knase 1; MKK4/6: Mitogen-activated protein kinase kinase 4/6; MyD88: Myeloid differentiation primary response protein 88; Mtor: Mechanistic target of rapamycin; NF-Κb: Nuclear factor kappa-B; PI3K: Phosphatidylinositol 3 kinase; PIM1: Proto-oncogene serine/threonine-protein kinase pim-1; p105: Nuclear factor NF-kappa-B p105 subunit; RAC1: Ras related C3 botulinum toxin substrate; Stat-1: Signal transducer and activator of transcription 1; SOCS-2/5: Suppressor of cytokine signaling; TAB1: TAK1-binding protein1; TAK1: TGF-beta activated protein kinase kinase 1; TIRAP: Toll-interleukin 1 receptor domain-containing adaptor protein; TLR2: Toll-like receptor 2; TLR4: Toll like receptor 4; TOLLIP: Toll interacting protein (├ direct inhibition); TRAF3: TNF receptor-associated factor 3; TRAF6: TNF receptor-associated factor 6; MAVS: Mitochondrial antiviral signaling protein that activates NF-kappa B and IRF 3; INFα/β: Interferon alpha/beta receptor; IRAK1–2: Interleukin receptor associated kinase 1, 2; IRF7: Interferon regulatory factor. P38MAPK: p38 mitogen-activated protein kinases; ECSIT: Evolutionarily conserved signaling intermediate in Toll pathways.
Figure 7.
Green ellipse indicates proteins identified in the present O. vulgaris library and blue ones indicate absence. (├ direct inhibition). AKT/PKB: RAC-alpha serine/thereonine-protein kinse/Protein kinase B; AIF: Apoptosis-inducing factor 1 mitochondiral; ATM: Ataxia telangiectasia mutated protein; BAX: Apoptosis regulator BAX. Bcl2: Apoptosis regulator Bcl-2; Bcl-XL: Bcl-2 like protein 1; BI1: BAX inhibitor-1; Casp 3, 6, 7, 8, 10: Caspase 3, 6, 7, 8, 10; Cytc: Cytochrome C; DFF40, 45: DNA fragmentation factor of 40 kD, 45 kD; FADD: FAS-associated via death domain; IAP: Inhibitor of apoptosis; IKK: Inhibitor of nuclear factor kappa-Bkinase; IκBα: MyD88: Myeloid differentiation primary response protein MyD88; NF-kappa-B inhibitor alpha. IL3R: Interleukin 3 receptor; NFκB: Nuclear factor kappa-B; PI3K: Phosphatidylinositol 3-kinase; p53: Tumor suppressor p53. RIP1: Receptor interacting serine/threonine-protein kinase1; TRADD: TNF receptor superfamily 1 alpha-associated via death domain; TRAF2: TNF-receptor-associated factor 2; TRAIL: TNF-related apoptosis-inducing ligand; Apaf1: Apoptotic protease-activating factor; FLIP: FADD-like apoptosis regulator; PTEN: Phosphatidylinositol-3, 4, 5-trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN; Smac: Second mitochondria-derived activator of caspase; Chk1/2: Checkpoint kinases 1, 2.
Figure 8.
Fold change in gene expression analysis by RT-qPCR.
Tissue expression profiles of immune genes in O. vulgaris. Data represent the fold change in expression of the analyzed transcripts relative to β-acting transcript level of sick octopuses (highly infected by A. octopiana), referred to healthy octopuses (null or lowly infected by A. octopiana). Results are mean ± standard deviation. Asterisks denotes significant differences (P<0.05).
Table 2.
Primer sequences used for RT-qPCR.