Figure 1.
Plastid genome maps of seven newly sequenced diatom species.
Species that share the same circular map have the same gene order. Genes on the outside are transcribed clockwise; those on the inside counterclockwise. The ring of bar graphs on the inner circle display GC content in dark grey.
Figure 2.
Phylogeny of Thalassiosirales and other diatom species based on twenty plastid protein-coding genes with gene/intron loss and plastid genome rearrangement events mapped on the branches.
Number of genome inversions within Thalassiosirales were estimated based on Thalassiosirales ancestral genome using GRIMM [29]. Taxa in bold are new genomes sequenced in this study.
Figure 3.
Comparison of inverted repeat boundaries in the seven diatom species newly sequenced for this study plus the two previously sequenced Thalassiosirales.
Tree is that of Figure 2 with previously sequenced outgroup taxa pruned for visual simplicity. The numbers in brown indicate plastid genome size; the numbers in black below each genome fragment indicate the sizes of the LSC, IR and SSC, respectively. Protein coding genes at the IR boundaries are listed in blue. Three red gene blocks are rrn5, rns and rnl, respectively. Names in bold are Thalassiosirales. Underscored names are for taxa newly sequenced for this study.
Figure 4.
Gene order comparison of the plastid genomes of seven diatoms sequenced for this study plus previously sequenced Thalassiosirales.
Alignments were performed in Geneious R6 [24]with mauveAligner [28]. Taxon names in bold are members of the Thalassiosirales. Names underscored are those sequenced for this study.