Table 1.
Number of individuals, size ranges, coelomocyte characterization, and cell viability after 24-hours recovery from highest levels exposures to UV-C and H2O2 of all echinoderms tested.
Figure 1.
Dose/concentration response in echinoderm coelomocytes.
Increase in DNA damage (strand scission factor, SSF, Fast Micromethod) with increasing concentration of H2O2 (A, C, E, and G) or dose of UV-C (B, D, F, and H) after acute exposure of coelomocytes from T. ventricosus (A and B, n = 5), L. variegatus (C and D, n = 11–12), E. l. lucunter (E and F, n = 6–7), and I. badionotus (G and H, n = 8). Data are means ± s.e.m.
Figure 2.
DNA repair in echinoderm coelomocytes.
DNA repair [reduction in DNA damage (SSF)] over a 24-hour period of recovery after acute exposure to H2O2 (A, C, E, and G) or UV-C (B, D, F, and H) in coelomocytes from T. ventricosus (A and B, n = 5), L. variegatus (C and D, n = 12), E. l. lucunter (E and F, n = 8), and I. badionotus (G and H, n = 8). Data are means ± s.e.m. *Significantly higher than controls, indicating incomplete repair (within 24-hour timepoint, Fisher's LSD, p<0.05).
Table 2.
Percent DNA repair (DNA damage at 24 hours recovery compared with initial DNA damage†) in echinoderm coelomocytes after 24 hours recovery from acute exposure to H2O2 or UV-C.