Figure 1.
Flow cytometric analysis of DNA replication in wheat anthers during early meiosis in the presence of the Ph1 locus.
a) Flow cytometric histograms of the nuclear DNA content of isolated anther nuclei in each stage of meiosis, which was determined by the number and organisation of centromeres (green) and telomeres (red) using fluorescence in situ hybridisation. The percentage of cells in G0/G1, S and G2/M are in brackets. G0/G1 and G2/M phases are shown in red, S phase is shown in green and total histogram (measured as total nuclei) is shown in blue. b) Progression of the percentage of cells in G0/G1, S and G2/M phases during early meiosis.
Figure 2.
Flow cytometric analysis of DNA replication in wheat anthers during early meiosis in the absence of the Ph1 locus.
a) Flow cytometric histograms of the nuclear DNA content of isolated anther nuclei in each stage of meiosis, which was determined by the number and organisation of centromeres (green) and telomeres (red) using fluorescence in situ hybridisation. The percentage of cells in G0/G1, S and G2/M are in brackets. G0/G1 and G2/M phases are shown in red, S phase is shown in green and total histogram (measured as total nuclei) is shown in blue. b) Progression of the percentage of cells in G0/G1, S and G2/M phases during early meiosis.
Table 1.
Flow cytometric determination of the percentage distributions of wheat somatic nuclei in each phase of the cell cycle in the presence and in the absence of the Ph1 locus (Ph1+ and Ph1-, respectively).
Table 2.
Flow cytometric determination of the percentage distributions of nuclei from wheat anthers in each phase of the cell cycle during meiosis in the presence of the Ph1 locus.
Table 3.
Flow cytometric determination of the percentage distributions of nuclei from wheat anthers in each phase of the cell cycle during meiosis in the absence of the Ph1 locus.
Figure 3.
Comparison of the DNA content in wheat anthers in each phase of the cell cycle during the progression of meiosis, in the presence and in the absence of the Ph1 locus.
Each value represents the mean of 9 measurements at each meiotic stage. a) Percentage of cell numbers in each stage of the meiosis in G0/G1 phase of the cell cycle showing no differences in any stage due to the presence/absence of Ph1 locus. b) Percentage of cell numbers in each stage of the meiosis in S phase. Differences were found in pachytene in the presence and in the absence of the Ph1 locus. c) Percentage of cell numbers in each stage of the meiosis in G2/M phase. Differences were found in zygotene in the presence and in the absence of the Ph1 locus. d) Regression line of the values of the percentage of wheat nuclei represented in panel a. No differences were found for the slope of the line either in the presence or in the absence of the Ph1 locus. e) Regression line of the values of the percentage of wheat nuclei represented in panel b. The slope of the line for replication was higher (in absolute value) in the absence of the Ph1 locus which means that the rate of replication is higher in its absence. f) Regression line of the values of the percentage of wheat nuclei represented in panel c. The slope of the line was higher in the absence of the Ph1 locus which implies that new replicated cells appear faster than in the presence of the Ph1 locus.
Table 4.
Comparison of the percentage of nuclei from wheat anthers in each phase of the cell cycle during early meiosis in the presence and in the absence of the Ph1 locus (Ph1+ and Ph1-, respectively).