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Table 1.

Results of the ILD, CADM, and CONCATERPILLAR tests.

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Table 2.

Datasets used for phylogenetic analyses and model settings as determined in jModeltest 2.0 and MrModeltest 2.3 using Akaike Information Criterion (AIC).

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Figure 1.

The ML tree of gymnosperms constructed from combined LFY and NLY CDS sequences.

Numbers associated with branches are bootstrap percentages higher than 50%. The cycads were used as functional outgroups.

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Figure 2.

Comparison of ML trees of gymnosperms constructed using LFY + NLY sequences.

A and C, All three codon positions were used; B and D, 1st and 2nd codon positions were used. A and B, Angiopteris lygodiifolia was used as outgroup; C and D, The cycads were used as functional outgroups. Numbers associated with branches are bootstrap percentages of ML higher than 50% and Bayesian posterior probabilities greater than 0.90, respectively.

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Figure 3.

The ML trees of cycads inferred from sequence analysis of combined LFY and NLY sequences.

A, CDS; B, CDS+Intron. Numbers associated with branches are bootstrap percentages of ML and MP higher than 50% and Bayesian posterior probabilities greater than 0.90, respectively. Ginkgo biloba was used as outgroup in Fig. 3A.

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Figure 4.

ML trees of Podocarpaceae constructed from sequence analysis of combined LFY and NLY sequences.

A, CDS; B, CDS+Intron. Numbers associated with branches are bootstrap percentages of ML and MP higher than 50% and Bayesian posterior probabilities greater than 0.90, respectively. Araucaria heterophylla and Agathis robusa were used as outgroups in Fig. 4A.

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Figure 5.

Divergence times of gymnosperms estimated from combined LFY and NLY CDS sequences using BEAST.

A time scale is shown at the bottom. A–K indicate fossil calibration points. 1–10, A, D, E and G indicate some nodes of interest. Median ages of nodes are shown, with horizontal bars indicating the 95% highest posterior density intervals.

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Figure 6.

The ML trees of Taxaceae+Cephalotaxaceae constructed from CDS sequences.

A, combined LFY and NLY; B, LFY; C, NLY. Numbers associated with branches are bootstrap percentages of ML and MP higher than 50% and Bayesian posterior probabilities greater than 0.90, respectively. Taiwania cryptomerioides and Cunninghamia lanceolata were used as outgroups.

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Figure 7.

A lineage-through-time plot showing divergence time distribution of the gymnosperm genera.

The divergence times was based on the median ages of the nodes from the BEAST analysis (see Fig. 5).

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Figure 8.

Comparison of divergence times of the coniferous genera between the Southern and Northern Hemispheres.

A, boxplot comparison of all genera; B, dot plot comparison of each genus. The calculation of divergence times was based on the median ages of the nodes from the BEAST analysis as shown in Fig. 5.

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