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Figure 1.

SEM micrographs.

Although a few polishing micro-traces and marks were observed, all specimens had a generally featureless and smooth surface. Oxinium (a), Co-Cr-Mo (b), Ti-6Al-4V (c), cp-Ti (d), stainless steel (e) Original magnification ×3000 (Scale bar = 1 µm)

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Figure 1 Expand

Table 1.

Surface roughness.

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Table 1 Expand

Table 2.

Contact angles and Surface free energies.

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Table 2 Expand

Figure 2.

Digital optical micrographs.

Biofilm incubated for 6 hours were stained with 0.5% crystal violet (black area). Oxinium (a), Co-Cr-Mo (b), Ti-6Al-4V (c), cp-Ti (d), stainless steel (e) Original magnification ×450 (Scale bar = 100 µm)

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Figure 2 Expand

Figure 3.

SEM images of biofilm.

Oxinium (a), Co-Cr-Mo (b), Ti-6Al-4V (c), cp-Ti (d), stainless steel (e) Original magnification ×1000 (Scale bar = 10 µm)

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Figure 3 Expand

Figure 4.

Biofilm coverage rate (BCR).

Mean and standard deviation are shown. *p<0.05, **p<0.01

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Figure 4 Expand

Figure 5.

Absorbance value of crystal violet (CV) assay after 6 hours incubation.

Mean and standard deviation are shown.

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Figure 5 Expand

Figure 6.

Colony forming units as determined by viable cell count (VCC) after 6 hours incubation.

Mean and standard deviation are shown.

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Figure 6 Expand