Table 1.
Summary of factors affecting the viability of isolated hepatocytes.
Table 2.
Summary of factors affecting the yield of isolated hepatocytes.
Table 3.
Variables considered for statistical analyses.
Figure 1.
Donor characteristics that have significant relationships with the viability (%) of hepatocytes after linear regression analyses.
Figures show relationships between viability and (A) age, (B) gender or (C) body mass index (BMI). Values were deemed significant when P<0.05.
Figure 2.
Variables measured in the blood or serum that have significant relationships with the viability (%) of hepatocytes after linear regression analyses.
Figures show relationships between viability and (A) aspartate aminotransferase activity (GOT; U/L), (B) gamma-glutamyltranspeptidase activity (GGT; U/L), (C) alanine aminotransferase activity (GPT; U/L), (D) bilirubin (mg/dL) or (E) quick value (%). Values were deemed significant when P<0.05.
Figure 3.
Liver variables that have significant relationships with the viability (%) of hepatocytes after linear regression analyses.
Figures show relationships between viability and (A) fibrosis, (B) liver fat or (C) Ludwig score. Values were deemed significant when P<0.05. For the variable of Ludwig score, variables not sharing the same alphabet are significantly different, P<0.05.
Figure 4.
Tissue processing and cell isolation variables that have significant relationships with the viability (%) of hepatocytes after linear regression analyses.
Figures show relationships between viability and (A) warm ischemia ex vivo (min) or (B) cold ischemia (min). Values were deemed significant when P<0.05.
Figure 5.
Donor characteristics that have significant relationships with the yield (million hepatocytes/gram liver) after linear regression analyses.
Figures show relationships between yield and (A) age, (B) gender, (C) body mass index (BMI), (D) diabetes, (E) hyperuricemia or (F) chemotherapy. Values were deemed significant when P<0.05.
Figure 6.
Variables measured in the blood or serum that have significant relationships with the yield (million hepatocytes/gram liver) after linear regression analyses.
Figures show relationships between yield and (A) alkaline phosphatase activity (AP; U/L), (B) aspartate aminotransferase activity (GOT; U/L), (C) gamma-glutamyltranspeptidase activity (GGT; U/L), (D) alanine aminotransferase activity (GPT; U/L), (E) bilirubin (mg/dL), (F) partial thromboplastin time (PTT; s) or (G) quick value (%). Values were deemed significant when P<0.05.
Figure 7.
Liver variables that have significant relationships with the yield (million hepatocytes/gram liver) of hepatocytes after linear regression analyses.
Figures show relationships between yield and (A) cirrhosis, (B) liver fat, (C) liver fat (%) or (D) Ludwig score. Values were deemed significant when P<0.05. For the variables of Ludwig score, operation type and surgical indication, variables not sharing the same alphabet are significantly different, P<0.05.
Figure 8.
Operation variables that have significant relationships with the yield (million hepatocytes/gram liver) of hepatocytes after linear regression analyses.
Figures show relationships between yield and (A) surgical indication, (B) operation type, (C) warm ischemia in vivo (min) or (D) weight of resected liver (g). Values were deemed significant when P<0.05. Abbreviations; hepatocarcinoma (HCC), focal nodular hyperplasia (FNH), cholangiocarcinoma (CCC), hemihepatectomy right (HR), hemihepatectomy left (HL), segment resection (SR), atypical resection (AR), extended hepatectomy (EH), lobectomy (L) and liver transplantation (LT).
Figure 9.
Tissue processing and cell isolation variables that have significant relationships with the yield (million hepatocytes/gram liver) of hepatocytes after linear regression analyses.
Figures show relationships between yield and (A) warm ischemia ex vivo (min) or (B) weight of perfused liver (g). Values were deemed significant when P<0.05.
Table 4.
The number of replicates (N) and the P values obtained after linear regression of the individual variables listed below to viability (%) or yield (million hepatocytes/g liver) of isolated human hepatocytes.
Table 5.
The regression coefficients (β), P values and R2 numbers of variables after multivariate analyses for the dependent variable of viability (%) of isolated human hepatocytes.
Table 6.
The regression coefficients (β), P values and R2 numbers of variables after multivariate analyses for the dependent variable of yield (million hepatocytes/g liver) of isolated human hepatocytes.
Figure 10.
The model for calculating projected viability is appropriate.
(A) Residuals versus fitted plot. (B) Normal quantile plot. (C) Square root of the standardised residuals versus fitted plot. (D) Standardised residuals versus leverage plot.
Figure 11.
The model for calculating projected yield is appropriate.
(A) Residuals versus fitted plot. (B) Normal quantile plot. (C) Square root of the standardised residuals versus fitted plot. (D) Standardised residuals versus leverage plot.
Table 7.
Viabilities (%) and yield (million hepatocytes per gram liver) of isolated human hepatocytes obtained by various groups.