Table 1.
Experimental design of alkali ballast water treatment.
Figure 1.
The rarefaction curve of Ion Torrent sequence data.
OTUs were defined at 97% similarity cutoff. The figure depicts the comparison between control discharge and NaOH treated discharge samples. The breaking line was placed at around 11,000 (10,918) reads and OTUs were compared across samples when samples were rarefied at 11,000 (10,918) reads.
Table 2.
Summary of averaged diversity measurements for each treatment group rarefied at 10,918 reads.
Figure 2.
A UPGMA tree constructed using weighted UniFrac distance matrix among the ballast water samples.
“P” and “N” in sample ID denote for PMA processed and Non-PMA processed, respectively. The Pie charts depict microbial community structure at genus level for each group. The top ten most abundant genera were shown and others were lumped in others.
Figure 3.
PCoA plot depicting a comparison of microbial communities derived from Ion Torrent and pyrosequencing.
RDP multiclassifier with 60% threshold was used to identify microbial taxa in each sample at genus level. PCoA plot was generated using Bray-Curtis dissimilarity matrix. Taxa that had the relative abundance of 0.5% or greater at least one of the samples were used to generate the matrix. The values in the parentheses indicate the percentage of eigenvalues for each axis.