Figure 1.
P. infestans is more sensitive to capsidiol than P. capsici.
(A) Verification of capsidiol purity as tested by NMR spectroscopy (Nuclear Magnetic Resonance Spectroscopy). 1H NMR (CDCl3, 600 MHz) spectrum of capsidiol. NMR integrations of the diagnostic methyl doublet at δH 0.88 ppm (expansion) reveal a purity of greater than 98.8%. (*) Represents the impurity. (B) Growth inhibition assay of P. infestans and P. capsici after 10 days of exposure of mycelial plugs to capsidiol. Pink bar delineates the lowest concentration with an inhibitory effect and the red bar the concentration after which there is no longer growth. This experiment was performed 4 times.
Figure 2.
Capsidiol inhibits P. infestans growth reversibly.
(A) Growth inhibition assay of P. infestans after 10 days of exposure of mycelial plugs to capsidiol. (B) Restoration of growth after washing treatment. Green line indicates the point after which the washing treatment was applied. The experiment was performed 3 times. Picture was taken 10 days after the washing and 20 days after initial exposure to capsidiol.
Figure 3.
Capsidiol is not affecting P. capsici growth as severely as it does P. infestans.
(A) Dose response curves of P. infestans 88069td calculated at 4, 7 and 10 days for both Fluorescence intensity and OD600. (B) Dose response curves of P. capsici tdtom calculated at 4, 7 and 10 days for both Fluorescence intensity and OD600.
Figure 4.
Scatter plots correlating fluorescence intensity and capsidiol concentration.
The plots illustrate fluorescence intensity of P. infestans 88069td (A) and P. capsici tdtom (B) strains over time for a maximum of 10 days. The experiment was performed 3 times.
Figure 5.
Scatter plots correlating 0D600 and capsidiol concentration.
The plots illustrate growth of P. infestans 88069td (A) and P. capsici tdtom (B) strains over time for a maximum of 10 days. The experiment was performed 3 times.
Figure 6.
Growth behaviour of P. infestans 88069td, after 10 days of exposure to different capsidiol concentrations.
The experiment was performed 3 times.
Figure 7.
Growth behaviour of P. capsici tdtom, after 10 days of exposure to different capsidiol concentrations.
The experiment was performed 3 times.
Figure 8.
Different P. infestans isolates have different sensitivity to capsidiol.
Isolates are clustered according to their sensitivity, starting from the most sensitive to the least. Top row of wells of each isolate represents capsidiol treatment (capsidiol was dissolved in DMSO/Plich media) in µM and the lower row represents treatment with 1% (v/v) DMSO/Plich (negative control). The experiment was performed 3 times.
Table 1.
Provenance of Phytophthora samples.