Table 1.
List of the F. velutipes strains used in this study.
Figure 1.
Mycelial growth of wild-type and degenerate dikaryons of F. velutipes.
(A) Morphology of the mycelia of the wild-type (left) and degenerate strains (right) that were grown on MCM agar for 7 d. (B) Growth rates of the wild-type and degenerate strains were determined based on the diameter of the mycelia grown at 25°C. The error bars represent the mean standard deviations of triplicate samples.
Figure 2.
Growth of monokaryotic mycelia and test crosses in F. velutipes.
(A) Crossing table of the monokaryons derived from the wild-type or degenerate strains. (B) Morphology of the mycelia of the degenerate monokaryons (right) and their wild-type (left) counterparts. W1 and D1 (upper panel) and W4 and D4 (lower panel) have compatible relationships. (C) Mycelial morphology of hybrids from crosses between monokaryons of wild-type and degenerate strains. The growth rates of the monokaryon (D) and hybrid test crosses (E) were determined based on the diameter of mycelia grown at 25°C.
Figure 3.
Mycelial running and formation of fruiting bodies.
(A) Mycelial running of the wild-type and degenerate dikaryons and the hybrids of crosses between monokaryons of the wild-type (Fv1-5) and degenerate (Fv1-5d) strains grown on sawdust medium in bottle cultures for 35 d at 20°C after inoculation. (B) Morphologies of the fruiting bodies produced by the strains represented in the left panel. The experiments were performed using triplicate samples.
Figure 4.
PCR-amplification products of the RAPD and SCAR primers.
(A) RAPD electrophoretic profile of monokaryons of the wild-type and degenerate strains obtained using random primer OPD-05. The arrows indicate the polymorphic bands of the wild-type (W4) and degenerate monokaryons (D4). (B) Specificity of the FEDE primer set (SCAR marker). Wild: Fv1-5 (wild-type), De: Fv1-5d (degenerate). W1 to W6 are monokaryons from Fv1-5, and D1 to D6 are monokaryons from Fv1-5d. W1-W3: group a (AxBy), W4-W6: group b (AyBx), D1-D3: group ad (AxBy), and D4-D6: group bd (AyBx).
Figure 5.
Synteny analysis of the genomic regions of D4 and W1 specific to the degenerate strain.
(A) DNA fragment of the W4 and D4 genomic region corresponding to 1 to 10 kb of scaffold 59 from the F. velutipes monokaryotic strain (Mono3) obtained by PCR using the SCAR primer set (FEDE-F and -R). Wild: Fv1-5 (wild-type), De: Fv1-5d (degenerate). W1 to W6 are monokaryons from Fv1-5, and D1 to D6 are monokaryons from Fv1-5d. W1–W3: group a (AxBy), W4–W6: group b (AyBx), D1–D3: group ad (AxBy), D4–D6: group bd (AyBx). (B) DNA fragment of the W4 and D4 genomic region corresponding to 60 to 121 kb of scaffold 59 obtained by PCR using the SCAR primer set.