Table 1.
Key physico-chemistry and major ion composition of control/diluent and mine and synthetic seepage waters.
Figure 1.
Effect of mine (MS) and synthetic (SS) seepage on growth rate of Chlorella sp.
Results are normalized to the percent of control response, and are expressed as mean ± standard error (n = 3 per tested concentration). Mine seepage toxicity data from Harford et al. [8] are also plotted. Mean ± standard error (n = 3) control growth rates (doublings/day) for the present study were as follows: MS sample 1–1.71±0.015; SS sample 1–1.71±0.015; MS sample 2–1.59±0.023; SS sample 2–1.61±0.032.
Table 2.
Summary of the toxicity of mine (MS) and synthetic (SS) seepage water from SNM to Chlorella sp and Moinodaphnia macleayi.
Table 3.
Effect of unmodified synthetic seepage, synthetic seepage with Mn, Br and Sr added, and mine seepage on growth rate of Chlorella sp.
Figure 2.
Effect of NaCl and HCO3 on growth rate of Chlorella sp.
Results are expressed as mean ± standard error (n = 3 per tested concentration). Data for NaCl toxicity are pooled from two tests. Mean (% coefficient of variation) (n = 3) control growth rates (expressed as doublings/day) were as follows: NaCl–1.65 (1.82%) and 1.72 (2.69%); HCO3–1.65 (1.82%).
Figure 3.
Effect of NaCl and NaSO4 on the reproduction of M. macleayi.
Results are expressed as the mean ± standard error (n = 5 or 10 individuals). Data for NaCl toxicity are pooled from two tests.
Figure 4.
Effect of mine (MS) and synthetic (SS) seepage on 3 brood reproduction of Moinodaphnia macleayi.
Results are normalized to the percent of control response, and are expressed as mean ± standard error (n = 10 per tested concentration). Mine seepage toxicity data from Harford et al. [8] are also plotted. Mean ± standard error (n = 10) control offspring per adult and percent adult survival for the present study were as follows: MS–20±2.4, 80% survival; SS–34±2.3, 90% survival.
Figure 5.
Effect of unmodified synthetic seepage (SS) and synthetic seepage with various ion excluded (–Mg, –Ca, –SO4, and –HCO3) on 3 brood reproduction of Moinodaphnia macleayi.
The secondary axis shows the electrical conductivity of the individual waters. Data are pooled from two toxicity test and results are expressed as the mean ± standard error (n = 19–20 individuals, except for –HCO3 n = 10). * indicates the –SO4 treatment is statistically different from the SS treatment (P = 0.008).
Table 4.
Comparison of single salt toxicity estimates with observed effect of synthetic waters.