Figure 1.
Digital holographic microscopy of staurosporine induced G1 arrest and etoposide or colcemid induced G2/M arrest.
L929 cells treated with 20 nM staurosporine (STS) or 3 µM colcemid (COL) or 1 µM etoposide (ETO) for 24 h (untreated controls, C). A. Digital holographic microscopy images, artificially coloured, with increasing thickness shown as green<red<white. B. STS treatment has no significant effect on average cell numbers; however it decreases average confluence and average cell volume. COL treatment has no significant effect on average cell number, instead average cell confluence decreases and average cell volume increases. ETO treatment significantly lowers average cell numbers, average confluence and increases average cell volume. Images and histograms are representative of n3. All results are significant except # which indicates P>0.05 compared to controls.
Figure 2.
Flow cytometric measurement of staurosporine induced G1 arrest and colcemid or etoposide induced G2/M arrest.
L929 cells treated with 20 nM staurosporine (STS) or 3 µM colcemid (COL) or 1 µM etoposide (ETO) for 24 h (untreated controls, C). A. DNA histograms obtained from flow cytometry measurements show a shift in the G2/M to G1 cell cycle phase for STS treatment and reversed for COL or ETO, a G1 to G2/M shift. B. STS increases G1 phase population of cells and COL and ETO increases G2/M phase population of cells. Images and histograms are representative of n3. All results are significant, P≤0.05 compared to controls.
Figure 3.
Digital holographic microscopy of dose-dependent etoposide induced G2/M arrest.
L929 cells treated with 0.1–10 µM etoposide (ETO) for 24 h (untreated controls, C). A. Digital holographic microscopy images of a dose-dependent increase in the average cell volume. Images are artificially coloured, with increasing thickness shown as green<red<white. B. ETO reduces average cell number, decreases average cell confluence and increases average cell volume. Images and histograms are representative of n3. All results are significant except # which indicates P>0.05 compared to controls.
Figure 4.
Flow cytometric measurement of dose-dependent etoposide induced G2/M arrest.
L929 cells treated with 0.1–10 µM etoposide (ETO) for 24 h (untreated controls, C). A. DNA histograms obtained from flow cytometry measurements show a dose-dependent shift in the G1 to G2/M cell cycle phase. B. ETO increases G2/M phase population of cells and decreases S and G1 population of cells. Images and histograms are representative of n3. All results are significant except # which indicates P>0.05 compared to controls.
Figure 5.
MTS cell viability measurement of dose-dependent etoposide induced G2/M arrest.
L929 cells treated with 0.1–10 µM etoposide (ETO) for 24 h. A. MTS analysis show a dose-dependent shift in cell viability post-etoposide treatment as compared to controls. All results are significant except # which indicates P>0.05 compared to controls, n3.