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Figure 1.

Virtual cytoplasm model in a cubic box of length 406 Å.

Each protein is represented by a collection of spheres representing the volume and approximate shape of a particular macromolecule.

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Figure 2.

Model for the calculation of local volume fraction for the sphere i.

In this model, Rcut is 4 times radius of central sphere i, and j and k are spheres that lie completely inside and partially inside Rcut, respectively.

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Figure 3.

Scaling of the mean-squared displacement as a function of time.

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Figure 4.

Diffusion coefficient values of the particles corresponding to GFP, CheY and FabD plotted against time.

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Table 1.

Diffusion coefficient values for three proteins with and without considering hydrodynamic interactions (HI). SD represents standard deviation.

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Figure 5.

Correlation function of successive displacements (eq. 11) for different time lags (see text) ranging from 100 ns to 1000 ns.

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Figure 6.

Anomalous subdiffusion occurring over short time regimes, tending to normal Brownian motion at larger time differences.

Diffusion is subdiffusive anomalous for t<3 on the log scale. After this time, the diffusion is approximately "normal" Brownian motion.

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Figure 7.

Correlation function of successive displacements (eq. 11) for simulation with and without hydrodynamic interactions.

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Figure 8.

Correlation function of successive displacements (eq. 11) with and without repulsive interactions (both are without hydrodynamic interactions).

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Figure 9.

Correlation function of successive displacements (eq. 11) for two different repulsive force constants (both are with hydrodynamic interactions).

Inset shows the same figure for a smaller range of y-axis.

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Figure 10.

Correlation function of successive displacements (eq. 11) showing the effect of stationary and mobile crowders.

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