Table 1.
Peri-operative characteristics of the patients studied for APJ protein analysis.
Table 2.
Patients recruited for experiment to determine whether APJ mRNA expression in myocardium excised from cyanotic patients differs from control (acyanotic) patients.
Table 3.
Patients recruited for experiment to determine the effect of re-perfusing myocardium at different O2 concentrations in vitro on APJ mRNA expression.
Figure 1.
1a: Western blot analysis of APJ receptor expression. Upper blot shows APJ receptor expression in myocardium obtained from patients used in the study. The lower blot shows HSP 70 levels in the same patient samples Numbers identify individual patients. C is internal placental loading control added to all gels. 50 µg protein was loaded in all wells. Figure 1b: APP receptor analysis in relation to length of the surgical time. Box and whiskers plot shows APJ receptor in the myocardial samples collected at the start of surgery (<10 min) and samples collected after 10 min until the end of surgery.
Figure 2.
APJ receptor analysis for cyanotic and acyanotic groups.
Comparison of myocardial APJ receptor mRNA for the cyanotic (n = 7) and acyanotic (n = 5) patient groups shown in Table 2. Expression was calculated using the Comparative Ct method to give RQ of gene expression using the StepOne Software v2.1). Statistical analysis was performed using the Mann-Whitney U test. Data shown as box and whiskers plot.
Figure 3.
Comparison of myocardial APJ receptor mRNA for samples collected from patients shown in Table 3 then re-perfused at 2, 20 or 60% O2 for 4 h.
Baseline are samples analysed before the re-perfusion experiments started. Expression was calculated using the Comparative Ct method to give RQ of gene expression using the StepOne Software v2.1). Statistical analysis was performed using the Mann-Whitney U test. Data shown as box and whiskers plot.
Figure 4.
Comparison of myocardial APJ receptor mRNA for samples collected from patients shown in Table 3 then re-perfused at 2, 20 or 60% O2 for 4 h.
The same patients as for Table 3 were use but split into 2 groups (cyanotic and acyanotic). Expression was calculated using the Comparative Ct method to give RQ of gene expression using the StepOne Software v2.1). Statistical analysis was performed using the Mann-Whitney U test. Data shown as box and whiskers plot.