Figure 1.
Embryonic and larval stages during early development of L. vannamei.
(Z, M and P are modified from Hertzler PL, 2009). The developmental stages drawn in this figure include zygote, 4-cell, blastula, gastrula, limb bud embryo, larva in membrane, NI, NIII, NVI, ZI, ZII, ZIII, MI, MII, MIII and P1.
Figure 2.
Length distribution of all-unigenes after clustering unigenes in each group.
X axis represents sequence length intervals. Y axis represents the number of unigenes in each interval.
Table 1.
Summary of sequencing and assembly of the transcriptome from L. vannamei.
Figure 3.
Summary of homology search of all-unigenes against nr database.
(a) E-value distribution of the best blast hits; (b) Similarity distribution of the best blast hits; (c) Species distribution of the best blast hits.
Figure 4.
GO annotation of all-unigenes.
Unigenes with GO annotation were divided into three major categories: biological process, cellular component and molecular function.
Figure 5.
COG classification of all-unigenes.
Unigenes were classified into 25 function classes. The columns represents the number of unigenes in each class.
Table 2.
Development related pathways and annotated key nodes.
Figure 6.
HCA and PCA plots for five samples using FPKM value of unigenes.
(a) Hierarchical clustering analysis plot. The height represents the Euclidean distance; (b) Principal components analysis plot. X axis represents PC1 that explains 78.7% and Y axis represents PC2 that explains 11.2% of the total variability for gene expression.
Figure 7.
Statistics of differentially expressed unigenes between adjacent samples.
Red columns represent the number of up-regulated genes while green columns represent the number of down-regulated genes.
Table 3.
The top five most significantly enriched GO terms.
Table 4.
The top ten enriched KEGG pathways.
Figure 8.
Expression profiles of genes involved in specific functions.
The red-green heat maps were drawn according to FPKM value. The Gray units represent zero for FPKM value. (a) Heat map for nuclear hormone receptor E75; (b) Heat map for amylase; (c) Heat map for myosin heavy chain type a (partial); (d) Heat map for calcified cuticle protein.
Table 5.
Partial functional categories and genes involved in early development of L. vannamei.
Figure 9.
Expression profiles of four unigenes.
X axis represents the developmental stages. Columns and bars represent the means and standard error of relative expression levels from qPCR result (Y axis at left). Lines represent the FPKM value from transcriptome result (Y axis at right). Asterisks represent the up-regulated transitions of each unigene.
Table 6.
Information of primers used for real time PCR.