Figure 1.
FBP concentration-vasorelaxation curves of phenylephrine-preconstricted thoracic aorta rings from SHRs.
Dose dependent response to cumulatively increasing concentration of DVWY (A), FDART (B), VVG (C), FQ (D), VAE (E), and WTFR (F). Vasorelaxant rate is expressed as the percentage reduction of evoked contraction. Each data point and bar represent the mean ± S.E. (n = 3). *p<0.05, **p<0.01, versus pre-treatment tension, Student's t-test.
Table 1.
In vitro ACE-inhibitory activities (IC50) of FBPs.
Figure 2.
Tissue and plasma ACE activities in SHRs after treatment with FBPs.
Animals were treated with FBPs at a dose of 10 mg/kg BW. The levels of ACE activity in thoracic aorta (A), heart (B), liver (C), kidney (D), lung (E), and plasma (F) were determined 6 hours after administration. Data represent the mean ± S.E. (n = 6). *p<0.05, **p<0.01 versus the control group (purified water administration), Student's t-test.
Table 2.
Maximum systolic and diastolic blood pressure pressure-lowering values at 6 h after administration of FBPs to SHRs (10 mg/kg BW, n = 6).
Figure 3.
Angiotensin II production after incubation with angiotensin I and DVWY, FDART, FQ, WTFR, and captopril.
Angiotensin II production was measured in SHR aorta rings incubated in 0.2 µM angiotensin I with DVWY, FDART, FQ, WTFR, and captopril (positive control). Data represent the mean ± S.E. (n = 3). *p<0.05, **p<0.01 versus the negative control group (Krebs solution treatment), Student's t-test.
Figure 4.
Vascular tension changes after incubation with angiotensin I and DVWY, FDART, FQ, WTFR, and captopril.
Tension changes were measured using SHR thoracic aorta rings incubated in 0.2 µM angiotensin I with DVWY, FDART, FQ, WTFR, and captopril (positive control). Data represent the mean ± S.E. (n = 3). *p<0.05, **p<0.01 versus the negative control group (Krebs solution treatment) by Student's t-test.
Figure 5.
Correlation between suppression of Ang II production and vasoconstriction by DVWY, FDART, FQ, and WTFR.
Correlation analysis of suppressive effects of Ang II production (IC50) and vasoconstriction (EC50). Correlation coefficients (r) and two-tailed P values are given in the inserts.