Figure 1.
Chemical structure and main metabolic pathways of albendazole and metabolites in organisms (Gyurik et al. [8]).
Table 1.
Mass spectrum parameters of albendazole and metabolites.
Figure 2.
Total ion mass spectra of albendazole and metabolites.
ABZ, albendazole; ABZSO, albendazole sulfoxide; ABZSO2, albendazole sulfone; ABZSO2-NH2, albendazole amino sulfone. Adjacent columns with the same color are two fragment ions from the same target analyte.
Figure 3.
Representative UFLC-MS/MS MRM chromatogram of albendazole and metabolites.
Peaks with the same retention time and approximate color are fragment ions from the same target analyte. Retention times: ABZ, blue, 2.45 min; ABZSO, red, 2.01 min; ABZSO2, orange, 2.02 min; ABZSO2-NH2, green, 2.27 min.
Figure 4.
Representative UFLC-MS/MS MRM chromatograms of albendazole (m/z 266.100>234.100) in standard substance (A) and the selected sample (A′).
Signals from different fragment ions are in blue and red.
Figure 5.
Representative UFLC-MS/MS MRM chromatograms of albendazole sulfoxide (m/z 282.200>208.100) in standard substance (B) and the selected sample (B′).
Signals of different fragment ions are in blue and red.
Figure 6.
Representative UFLC-MS/MS MRM chromatograms of albendazole sulfone (m/z 298.200>159.100) in standard substance (C) and the selected sample (C′).
Signals of different fragment ions are in blue and red.
Figure 7.
Representative UFLC-MS/MS MRM chromatograms of albendazole amino sulfone (m/z 240.200>133.100) in standard substance (D) and the selected sample (D′).
Signals of different fragment ions are in blue and red.
Table 2.
Maximum permissible deviation of relative ion abundance.
Table 3.
Recovery (%), repeatability (RSD%), LODs and LOQs of albendazole and metabolites in QC samples (n = 6).
Table 4.
Intraday and interday precision and accuracy for quantification of albendazole and metabolites from silkworm samples (n = 6).
Table 5.
RSDs (%) of sample peak areas in stability test (n = 6).
Figure 8.
Albendazole, albendazole sulfoxide, albendazole sulfone and albendazole amino sulfone in silkworm hemolymph after feeding with albendazole-treated mulberry leaves.