Figure 1.
Comparison of MFGM proteins from the different TC and control lines.
Cloned and conventionally bred control animals [(C+N)] were compared with (A) MFGM proteins from the TC-LA line, (B) MFGM proteins from the TC-LF line, and (C) MFGM proteins from the TC-LZ line. (D) The intersection of differentially expressed MFGM proteins from the three TC groups yielded 43 proteins in common.
Figure 2.
Functional analysis of MFGM proteins specifically expressed in the transgenic cloned animals.
Analysis of proteins enriched in the transgenic cloned groups according to: (A) biological process, (B) cellular component, and (C) molecular function. Blue curves indicate the degree of enrichment of gene function.
Table 1.
Proteins identified in the MFGM of colostrum and mature milk of the TC-LZ, TC-LA, and TC-LF transgenic cloned lines and of the cloned and conventionally bred normal controls.
Table 2.
Proteins specifically expressed in transgenic cloned cattle MFGM.
Figure 3.
Cluster analysis of MFGM proteins.
(A) Analysis of MFGM proteins in colostrum of the three TC groups compared to cloned [(C)] and conventionally bred [(N)] controls. (B) Analysis of MFGM proteins in mature milk of the three transgenic groups compared to controls.
Figure 4.
Principal component analysis of MFGM proteins from the three transgenic groups, the cloned group, and the conventionally bred control group.
(A) Analysis of MFGM proteins in colostrum. (B) Analysis of MFGM proteins in mature milk.
Figure 5.
Molecular function analysis of differentially expressed MFGM proteins in the transgenic groups.
Enrichment analysis of differentially expressed MFGM proteins in (A) TC-LA colostrum, (B) TC-LA mature milk, (C) TC-LF colostrum, (D) TC-LF mature milk, (E) TC-LZ colostrum, and (F) TC-LZ mature milk. Blue curves indicate the degree of enrichment of gene function.
Figure 6.
Pathway analysis of differentially expressed proteins from the transgenic groups.
IPA defined pathways of the differentially expressed MFGM proteins from: (A) TC-LA colostrum, (B) TC-LA mature milk, (C) TC-LF colostrum, (D) TC-LF mature milk, (E) TC-LZ colostrum, and (F) TC-LZ mature milk.
Figure 7.
Molecular function and pathway analysis of differentially expressed MFGM proteins from the cloned control group.
(A, B): Molecular function of MFGM proteins from colostrum (A) and mature milk (B). (C, D): IPA defined pathways of MFGM proteins from colostrum (C) and mature milk (D).
Figure 8.
Pathway analysis involved in the Lipid Metabolisms of the TC-LA MFGM proteins.