Figure 1.
Flow cytometry gating strategy for HIV-1Tg and F344 rat blood.
Mononuclear, lymphocytes, and granulocytes were gated on FSC versus SSC. Cell surface antibodies were used to identify B and T cells, T cytotoxic cells, T helper cells, neutrophils, monocytes, and monocyte subtypes (classical and non-classical).
Figure 2.
Analysis of cell populations in whole blood from HIV-1Tg and F344 rats.
Flow cytometry analysis of untreated HIV-1Tg immune cell populations (red line) and untreated F344 age-matched rats (blue line). (A) T cells (CD3+); (B) T helper cells (CD3+/CD4+); (C) T cytotoxic cells (CD3+/CD8+); (D) B cells (CD45RA+); (E) neutrophils; (F) monocytes; (G) classical monocytes (CD43+); (H) non-classical monocytes (CD 43++). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 3.
Analysis of cell populations in whole blood from aging HIV-1Tg and F344 rats, with and without LPS treatment.
Flow cytometry analysis of HIV-1Tg cell populations (red bars) and F344 age-matched control rats (blue bars). Solid bars indicate control samples and bars with striped lines indicate samples that have been treated with LPS. (A) T cells; (B) T helper cells; (C) T cytotoxic cells; (D) B cells; (E) neutrophils; (F) monocytes; (G) classical monocytes; (H) non-classical monocytes. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 4.
Immune cells in the spleens of aging HIV-1Tg and F344 rats, with and without LPS treatment.
Flow cytometry analysis of immune cells from the spleens of HIV-1Tg rats (red bars) and F344 age-matched control rats (blue bars). Solid bars indicate control samples and bars with striped lines indicate samples that have been treated with LPS. (A) T cells; (B) T helper cells; (C) T cytotoxic cells; (D) B cells; (E) neutrophils; (F) monocytes; (G) non-classical monocytes; (H) classical monocytes. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 5.
Cytokines and chemokines in the serum of aging HIV-1Tg and F344 rats, with and without LPS treatment.
Cytokine and chemokine levels in serum from HIV-1Tg rats (red bars) and F344 age-matched control rats (blue bars). Solid bars indicate control samples and bars with striped lines indicate samples treated with LPS. (A) IL-2; (B) IFN-γ; (C) IL-4; (D) IL-13; (E) IL-5; (F) IL-10; (G) IL-1β; (H) KC/GRO; (I) IL-6; (J) TNF-α. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 6.
Cytokines and chemokines in the spleens of aging HIV-1Tg and F344 rats, with and without LPS treatment.
Cytokine and chemokine levels in the spleen of HIV-1Tg rats (red bars) and F344 age-matched control rats (blue bars). Solid bars indicate control samples and bars with striped lines indicate samples treated with LPS. (A) IL-2; (B) IFN-γ; (C) IL-4; (D) IL-13; (E) IL-5; (F) IL-10; (G) IL-1β; (H) KC/GRO; (I) IL-6; (J) TNF-α. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 7.
IL-6 and TNF-α in lymph nodes of aging HIV-1Tg and F344 rats, with and without LPS treatment.
Pro-inflammatory cytokines, (A) IL-6 and (B) TNF-α, were examined in the lymph nodes of HIV-1Tg (red bars) and age-matched F344 control rats (blue bars), with and without LPS treatment. Solid bars indicate control samples and bars with striped lines indicate samples treated with LPS. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Figure 8.
IL-6 and TNF-α in the spleens of aging HIV-1Tg and F344 rat spleen, with and without LPS treatment.
The integrated intensity of TNF-α and IL-6 protein from the spleens of HIV-1Tg (red bars) and F344 age-matched control rats (blue bars), with and without LPS treatment, was measured using Western blot analysis and normalized to β-actin protein. (A) IL-6 after saline treatment; (B) IL-6 after LPS treatment; (C) TNF-α after saline treatment; (D) TNF-α after LPS treatment. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.