Figure 1.
Species delimitation decision-making workflow.
Symbols to the left of each step represent the different classes of data used at that particular step: star = morphological data; circle = mitochondrial data (COI); square = nuclear data.
Figure 2.
Geographic sampling of the nondimorphicus group.
Localities are those from recent fieldwork. Localities with “T” correspond to type localities.
Figure 3.
Geographic sampling of the cavicolens and robustus groups.
Localities are those from recent fieldwork. Localities with “T” correspond to type localities. Type localities with solid circles correspond to localities sampled during this study.
Figure 4.
Multivariate clustering results.
Left panel: specimens grouped by delimited clusters. Right panel: specimens colored by species (both previously and newly described).
Table 1.
Nuclear gene data alignment and diversity statistics.
Figure 5.
*BEAST species tree with BPP species delimitations.
Numbers below nodes on species tree correspond to posterior probability support. Earliest diverging braches shortened for graphical purposes. At bottom left, BPP results with 4 different prior combinations. Numbers correspond to results of the two separate BPP runs. Red letters correspond to those nodes on the species tree.
Table 2.
Results of gsi analyses.
Figure 6.
Results of integrative species delimitation.
Solid color boxes indicate successful identification or delimitation of a species for the particular approach. For the morphometric clustering method, connected boxes indicate clusters including specimens from multiple species within the same species group. Clusters including specimens from multiple species from different species groups are indicated with multicolor boxes. For the mtDNA analyses [22], the vertically striped box for S. glorietus indicates paraphyly. For S. steinmanni, only one specimen was sequenced, so mitochondrial monophyly could not be assessed. For BPP analyses, asterisks indicate that S. ungulatus and S. madhousensis were not supported in analyses with certain prior combinations (see Results). Only species with gsiT values greater than 0.8 are indicated.
Figure 7.
Comparative penis morphology for Sclerobunus species groups.
A) cavicolens group - lateral and ventral views, S. ungulatus (Model Cave, NV). B) nondimorphicus group - lateral and ventral views, S. idahoensis (Meadow Creek, ID), arrow indicates smooth dorsal surface of the ventral plate. C) robustus group - lateral and ventral views, S. robustus (Chiricahua Mountains, AZ), arrow indicates dorsal surface of the ventral plate with many folds. DP = dorsal plate, LP = lateral plates, VP = ventral plate. Scale bars = 0.25 mm.
Figure 8.
Comparative penis morphology for S. speoventus and S. steinmanni.
A) lateral and ventral views, S. speoventus, paratype (Cave of the Winds, CO), arrow indicates curved subapical spines. B) lateral and ventral views, S. steinmanni, holotype (Mallory Cave, CO), arrow indicates dorsoventrally compressed ventral plate. Scale bars = 0.25 mm.
Figure 9.
Habitus morphology of the nondimorphicus and cavicolens groups.
A) S. cavicolens, male, image horizontally reflected (Lewis and Clark Caverns, MT) [835849]. B) S. ungulatus, male (Model Cave, NV) [835874]. C) S. madhousensis, male (North Madhouse Cave, UT) [835858]. D) S. nondimorphicus, male, image horizontally reflected (Iron Creek, WA) [835861]. E) S. idahoensis, male (Hobo Cedar Grove, ID) [835854]. Scale bars = 1 mm. Morphbank numbers indicated in brackets.
Figure 10.
Habitus morphology of the robustus group.
A) S. speoventus, paratype male (Cave of the Winds, CO) [835868]. B) S. steinmanni, holotype male (Mallory Cave, CO) [835871]. C) S. robustus male (Apex Valley, CO) [835865]. D) S. glorietus, male (Glorieta Canyon, NM) [835850]. E) S. klomax, holotype female (Taos Ski Valley, NM) [835857]. F) S. jemez, holotype male (Terrero Cave, NM) [835856]. G) S. skywalkeri, paratype male, image horizontally reflected (Manzano Mountains, NM) [835867]. Scale bars = 1 mm. Morphbank numbers indicated in brackets.