Table 1.
Species of Sorghum included in the study. Chromosome numbers are based on the literature review.
Table 2.
Primer sequences and PCR protocols in the study.
Table 3.
Sequence and tree statistics for LCN and plastid genes used in this study.
Figure 1.
Maximum likelihood phylogeny of Sorghum inferred from nuclear Pepc4 data.
Numbers above branches are maximum likelihood bootstrap/Bayesian posterior probability (MLBS/PP). Taxon labels are in the format: Sorghum brachypodum-2-Cowie8981-×2 where Sorghum brachypodum indicates that the sequence belongs to the species Sorghum brachypodum; -2- = the second sequence listed in Table S1 for the species; Cowie8981 = specimen voucher information; -×2 indicates we recovered 2 clones for the sequence; and without any mark after specimen voucher information indicates the sequence is derived from PCR-direct sequencing. Coloured taxon labels and circles correspond to the listed subgenera and geographic ranges at the top left corner of the figure, respectively.
Figure 2.
Maximum likelihood phylogeny of Sorghum inferred from nuclear GBSSI data.
Numbers above branches are maximum likelihood bootstrap/Bayesian posterior probability (MLBS/PP). Taxon labels are in the format: Sorghum matarankense-2-Perry2691-×3 where Sorghum matarankense indicates that the sequence belongs to the species Sorghum matarankense; -2- = the second sequence listed in Table S1 for the species; Perry2691 = specimen voucher information; ×3 indicates we recovered 3 clones for the sequence; and without any mark after specimen voucher information indicates the sequence is derived from PCR-direct sequencing. Coloured taxon labels and circles correspond to the listed subgenera and geographic ranges at the top left corner of the figure, respectively.
Figure 3.
Chronogram of Sorghum and relatives based on three plastid sequences (ndhA intron, rpl32-trnL, and rps16 intron) as inferred from BEAST.
Numbers above the branches are maximum likelihood bootstrap/Bayesian posterior probability (MLBS/PP). Taxon labels are in the format: Sorghum almum-Liu236 where Sorghum almum indicates that the sequence belongs to the species Sorghum almum; -Liu236 = specimen voucher information. Coloured taxon labels and circles correspond to the listed subgenera and geographic ranges at the top left corner of the figure, respectively. Numbers 1–9 indicate the lineages of interest as shown in Table 4.
Table 4.
Posterior age distributions of lineages of interest in Sorghum plus Cleistachne sorghoides.
Table 5.
A proposed new subgeneric classification of Sorghum Moench (subtribe Sorghinae Clayton & Renvoize, tribe Andropogoneae Dumort.) based on plastid and nuclear DNA data (*not examined in this study).