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Figure 1.

Phylogenetic analysis of amino acid sequences of a partial sequence of Chsp.

Tree inferred from conserved motifs (CON1S) with the ML method using the evolutionary model LG. Bootstrap consensus tree, inferred from 1000 replicates, to represent the evolutionary history of the taxa analyzed. (A) Condensed tree showing topology of the classes' distribution among the analyzed phyla. (B) Circular topology representation including all sequences identified.

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Table 1.

Chsp Class specific Regular Expressions.

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Figure 2.

Chsp distribution in fungal species diagram.

Phylogeny of 54 species of fungi. Bars indicate the class and copy number of putative Chsp in the selected genomes. Chsp previously reported but not found on this study are labeled as “missing.” Phylogeny tree modified from [33].

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Figure 3.

ChspIV phylogenies.

Phylogenetic trees inferred using the methods: (A) ME, (B) NJ, (C) MP, (D) ML, and (E) Bayesian. The triangles of the same color represent groupings of the same putative Chsp sequences.

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Figure 4.

Syntenic analysis of SynA_1.

Genomic blocks (Locally Collinear Blocks or LCBs, shown as colored boxes) from (A) Neosartorya fischeri, (B) Aspergillus fumigatus, (C) A. clavatus, (D Emericella nidulans, (E) A. oryzae, and (F) A. terreus. Vertical crisscrossing lines indicate orthology. Same color on gene names indicate functional relation. Arrow heads indicate direction of transcription.

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Figure 5.

Gene expression correlation for putative cell-wall metabolism clutster.

Hierarchical cluster dendrogram showing the correlations among gene expression for members of the putative cell-wall metabolism gene cluster on Aspergillus oryzae (NCBI GEO GSE9298) [37]. 36 probes (genes) were analyzed: the putative ChspIV plus 15 upstream and 20 downstream. Correlations are based on a dissimilarity measure of (1-r2) in which correlation values are assigned “distance” values ranging from 0.0 (completely correlated, r2 = 1) to 1.0 (completely uncorrelated, r2 = 0). The y-axis represents the height or distance between the gene groups divided at that point.

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