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Figure 1.

Schematic illustration of the simulation procedure.

(a) Corneal geometrical data from Scheimpflug images are used to define the model geometry. Inverse modeling is performed to account for the effect of applying the IOP. (b) The temporal pressure profile measured experimentally and the spatial pressure profile obtained from CFD (Computation Fluid Dynamics) simulation are applied to the cornea as a function of time, location and current deformed shape. (c) The finite element model is solved for the current parameter set and simulation results are compared to the experimentally measured deformation. A step-wise optimization approach is used to find the parameter set that leads to the most similar deformation.

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Figure 2.

Correlation between the peak distance and apex indentation obtained from an ample experimental data set, including corneal response under different stiffness, thickness and IOPs.

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Figure 3.

Geometry model of the cornea at maximal deformation.

Mesh of cells of the modeled air volume (left) and streamlines (right) colored by the flow velocity distribution.

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Table 1.

Geometrical parameters of deformed corneas and the corresponding mesh size.

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Figure 4.

Spatial pressure distribution of the air-puff along the corneal surface for different deformed shapes.

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Figure 5.

Air-puff characterization.

(a) Experimentally measured temporal air-puff profile; (b) Results from CFD simulation showing the air-puff as a function of apex indentation and location along the cornea (horizontal distance from the apex).

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Table 2.

Biomechanical and geometrical model parameters used to simulate the human and pig corneal deformation.

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Figure 6.

Schematic of the optimization process.

Pre-optimization step (0), where the maximal air-puff pressure is adjusted; Multi-step optimization, comprising adjustment of initial elastic modulus and corneal geometry (I); adjustment of viscoelastic parameters (II) and further refinement of the elastic modulus and geometry (I); adjustment of the anterior and posterior elastic moduli (III), followed by a further refinement of the elastic moduli and geometry (I). In human eyes and additional step was incorporated to account for damping by ocular muscles and external tissue (IV). In the illustration the size of the loops is positively correlated with the dominance of the parameter adjusted therein. The resulting parameter values, shown in blue, represent an example of the output parameters at each step for human eyes ex vivo and in vivo.

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Figure 7.

Effect of the change of different biomechanical parameters on the spatial deformation profiles (upper row) and temporal deformation profiles (lower row) at IOP = 15 mmHg.

(a) Elastic properties dominate the maximal indentation depth. (b) Viscoelastic properties dominate the amount of hysteresis when the air-pressure has decreased to zero. (c) The ratio between anterior and posterior stiffness dominates the distance between corneal apex and bending points.

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Figure 8.

Temporal (a, c) and spatial (c, d) corneal deformation with air-puff.

Dotted lines represent experimental corneal deformations and continuous lines simulated corneal deformations Panels (a, b) show data for porcine corneas: simulated and experimental data at different IOPs. Panels (c, d) show data for human corneas: simulated response with and without ocular muscle damping, compared to in vivo experimental deformations measured in patients and ex vivo deformations measured in an enucleated whole globe.

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Table 3.

Corneal biomechanical parameters obtained from finite element analysis: Elasticity modulus represents the static material properties; The difference between anterior and posterior cornea describes the differences in collagen interweaving and the resulting higher anterior corneal rigidity; The relaxation time – which must lie within the temporal scale that was analyzed – describes the point of time at which the corneal stiffness decreased by the factor of the relative modulus; The muscle spring and damping constants describe the static and dynamic displacement, respectively, due to whole the eye movement in in vivo measurements.

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Table 4.

Parameter gradient from the sensitivity analysis.

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