Table 1.
Clinical characteristics of participants.
Figure 1.
Flowchart illustrates the processing algorithm used for the analyses. VBM-based spatial preprocessing is combined with disease-independent intensity normalization to allow for maximal standardization and direct comparability of T1-contrasted MRI images.
Figure 2.
Group comparison of ALS patients versus healthy controls.
Wholebrain group-mean of VBI images projected on generic brain. Coronal (a), sagittal (b) und axial (c) sections illustrate significantly higher intensity in the CST, corpus callosum and posterior limb of the internal capsule (PLIC). Significant VBI increases were see descending from the motor-cortical level (d) into the cerebral peduncle, rendering areas like the radiate corona (e), PLIC (f) to the midbrain nuclei (g). Inference was done using Threshold Free Cluster Enhancement (10000 permutations) und Family-wise error rate correction for multiple comparisons. Color spectrum gives p-value indication (h).
Table 2.
Intensity response in key CST areas and control regions.
Figure 3.
Group comparison of ALSFRS-R stratified patient subgroups versus healthy controls.
Wholebrain group-mean of VBI images projected on generic brain (a, b) and Maximum Intensity Projection of significantly different regions (d, e). The extent of MRI intensity change in ‘low’ disability group (a, d) is significantly less widespread than in the ‘high’ disability group (b, e). Inference was done using Threshold Free Cluster Enhancement (10000 permutations) und Family-wise error rate correction for multiple comparisons. Color spectrum giving p-value indication (c).
Figure 4.
Regression analysis of VBI-processed T1 intensities of all ALS patients and their ALSFRS-R.
Clusters of significant correlation between VBI intensity and ALSFRS-R scores are given in coronal slices (a, level at b). Projection on VBI group mean generic brain, CST highlighted according to JHU DTI atlas (yellow). Mean intensity inside ROIs in the PLIC (f, 5 mm sphere), paraventricular (e, 10 mm sphere) and subcentral white matter (d, 10 mm sphere) reveals overall decrease in intensity (mean VBI intensity versus ALSFRS-R; g, right; h, left). This illustrates a disability-related MRI intensity change in ‘core’ regions of ALS-related white matter disturbances. Inference was done using Threshold Free Cluster Enhancement (10000 permutations). Color spectrum giving p-value indication (c).
Figure 5.
Group comparison of bulbar and limb phenotype patient subgroups versus healthy controls.
Wholebrain group-mean of VBI images projected on generic brain (c) and Maximum Intensity Projection (MIP) of significantly different regions (d, e, f). The patterns of MRI intensity change in bulbar (a) and limb (b) subgroups significantly differ despite no significant differences in age, sex or ALSFRS-R score between groups. Coronal (d), sagittal (e) and axial (f) MIPs illustrate apparently more widespread involvement of cerebral white matter in bulbar-onset ALS. Inference was done using Threshold Free Cluster Enhancement (10000 permutations) und Family-wise error rate correction for multiple comparisons. Color spectrum giving p-value indication (g).