Figure 1.
Acylation of ara-C with vinyl laurate catalyzed by whole-cells (1: Ara-C; 2: VL; 3: Unstable enol; 4: Aldehyde; 5: 3′-O-lauryl ara-C; 6: 5′-O-lauryl ara-C).
Table 1.
Acylation of ara-C with vinyl laurate by various bacteriaa.
Table 2.
Acylation of ara-C with vinyl laurate by P. fluorescens in different organic solvent systemsa.
Figure 2.
Solvent-sensitivity of the whole-cells in organic solvents (The whole-cells were kept in different mixture organic solvents under 30°C for 6 h before catalyzed the reaction).
Figure 3.
SEM photographs of freeze-dried P. fluorescens cells after incubated in organic solvents for 24 h {a: PBS, b: DMF, c: IPE/pyridine (v/v = 1∶3), d: THF/pyridine (v/v = 1∶3)}, magnification was 10 K, scale bar: 1 µm.
Table 3.
Effects of organic solvents on cell growth of P. fluorescens.
Figure 4.
SEM photographs of freeze-dried P. fluorescens grown in the presence of 1% different organic solvents (a: water, b: Pyridine, c: DMSO, d: IPE), magnification was 10 K.
Circles: b–cotton-like debris, c–sags, scale bar: 1 µm.