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Table 1.

Overview of MRSA clone characteristics, site of isolation, and biofilm formation in static and shear flow assays.

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Figure 1.

Box-whisker plot of biofilm formation by MRSA clones in the static assay.

Boxes depict the 95% CI, black horizontal lines the average OD492 value, and the whiskers the OD492 value range (lowest and highest values) for each clone.

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Figure 2.

Performance of MRSA clones in the static and shear flow assay.

A) In the static assay: biomass production was subdivided into weak, moderate and strong. B) In the shear flow assay biofilm formation was assessed as positive or negative.

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Figure 3.

Live/dead BacLight staining and CLSM on 72-hour old MRSA biofilms under shear flow (Bioflux system).

A) 3-dimensional representations. B) 2-dimensional projections, thin white lines in the xy plane depict the location of the section plane on the z axis. White bars have a length of 10 µm.

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Figure 4.

Space occupied by live (green) or dead (red) or total (grey) cells by different epidemic MRSA clones in a biofilm.

Error bars denote standard deviations were calculated using 1 (USA300, EMRSA-15), 2 (USA600, Hungarian/Brazilian, Iberian) or 3 (USA500) independent z-stacks.

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Figure 4 Expand