Figure 1.
Diagram reflecting the experimental design and sampling points employed in the study of the transcriptomic profiles associated to the vaccination and VHSV infection.
This experimental prodecure is explained in detail in the materials and methods section.
Figure 2.
qPCR validation of the microarray data.
Correlation between microarray (x-axis) and qPCR (y-axis) data (Log10 fold-change) from 5 genes at 3 different times after infection of vaccinated and unvaccinated turbots. Fold-change values of the selected genes were displayed in Table S1. The correlation between microarray and qPCR data analyzed by the Spearman’s Rho test was ρ = 0.969 and with a statistical significance of p<0.01.
Figure 3.
Evolution of viral genes transcription in non-vaccinated and vaccinated turbot.
Sequences for the five proteins encoded by VHSV were included in the microarray design for analyzing their evolution in non-vaccinated (PBS – VHSV and pMCV1.4– VHSV) and vaccinated (pMCV1.4-G860– VHSV) individuals at 8, 24 and 72 h post h post-challenge.
Figure 4.
Stacked column chart reflecting the distribution of regulated genes through time after pMCV1.4 or pMCV1.4-G860 intramuscular injection.
Statistically significant differential gene expressions are subdivided according to intensity (fold change) and sense (up and down-regulation).
Figure 5.
Stacked column chart reflecting the distribution of regulated genes through time after VHSV challenge in non-vaccinated (PBS – VHSV or pMCV1.4– VHSV) and vaccinated (pMCV1.4-G860– VHSV) turbot.
Statistically significant differential gene expressions are subdivided according to intensity (fold change) and sense (up and down-regulation).
Figure 6.
Heat map displaying hierarchical clustering results from microarray expression data of genes implicated in the Toll-like receptor signalling pathway.
All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.
Figure 7.
Heat map displaying hierarchical clustering results from microarray expression data of genes involved in the IFN system (IFNs, IRFs, ISGs…).
All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.
Figure 8.
Heat map displaying hierarchical clustering results from microarray expression data of genes involved in the (A) apoptosis pathway and in the (B) cell death induced by cytotoxic cells.
All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.
Figure 9.
Heat map displaying hierarchical clustering results from microarray expression data of genes involved in the MHC-I antigen presentation process.
(A) Enzymes involved in the ubiquitination of target proteins. (B) Subunits belonging to the proteasome complex. (C) MHC class I related-proteins and molecules implicated in the antigen-MHC class I assembly. All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.
Figure 10.
Heat map displaying hierarchical clustering results from microarray expression data of genes belonging to the (A) coagulation or (B) complement cascades.
All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.
Figure 11.
Heat map displaying hierarchical clustering results from microarray expression data of gene markers of activation and proliferation of cell types.
All the experimental groups, including the corresponding controls (PBS 8 h and PBS – MEM 8 h), were included in the analysis. Different genes are represented in different rows, and different experiments in different columns. Raw expression values are represented as a color scale from red for lower expressions to green for higher expressions.