Table 1.
Composition and nutrient levels of the basal diet (DM basis) %.
Table 2.
Sequences (5′–3′) of the primers used for the detection of mRNA specific for IL-1β, IL-6, TNF-α and GAPDH.
Figure 1.
Changes in diarrhea index in piglets.
Piglets challenged by enterotoxigenic Escherichia coli were fed either a control diet (A group, n = 10), or control diet plus chlortetracycline (B group, n = 10) or control diet plus chitosan (C group, n = 10) for 21 days. Diarrhea index (%) was calculated as 100× number of piglets that had diarrhea/total number of piglets.
Table 3.
Serum IL-1β, IL-6 and TNF-α levels in weaned piglets.
Figure 2.
Calprotectin and TLR4 protein expression in jejunal mucosa (immunohistochemical staining, ×400).
Piglets challenged by enterotoxigenic Escherichia coli were fed either a control diet (A group, n = 6), or control diet plus chlortetracycline (B group, n = 6) or control diet plus chitosan (C group, n = 6) for 21 days and 6 pigs per treatment were killed to excise the jejunum.
Table 4.
Integral optical density of Calprotectin and TLR4 protein expression in jejunal mucosa.
Figure 3.
The relative expression of IL-1β, IL-6 and TNF-α mRNA in jejunal mucosa.
Piglets challenged by enterotoxigenic Escherichia coli were fed either a control diet (A group, n = 6), or control diet plus chlortetracycline (B group, n = 6) or control diet plus chitosan (C group, n = 6) for 21 days and 6 pigs per treatment were killed to excise the jejunum.