Figure 1.
Section of a valve of a P. margaritifera shell.
(a) dorso-ventral side showing the transition areas between nacreous and prismatic layers. (b) dorsal part; (c) ventral part; (d) central part; (e) detail of ventral part. (1) prismatic calcite; (2) aragonite tablets. The red arrows indicate the calcein mark.
Table 1.
Set of forward and reverse primers used for the gene expression analysis.
Figure 2.
Shell deposit rate (µm.d−1) on the ventral side of the shell according to temperature and food concentrations over the two month experiment.
Three temperatures (21, 25 and 28°C) and two microalgal concentrations (800 cell mL−1: light grey and 15000 cell mL−1: darkgrey) were tested. The five homogeneous groups identified by the Tukey post-hoc test (a, b, c and d) are indicated (means and standard error, n = 10).
Figure 3.
Relative gene expression of genes coding for proteins potentially involved in the construction of the prismatic layer (KRMP 7, mantle protein 10, shematrin 9, MPN88, fibronectin 1) the nacreous layer (MSI60, Pif 177, pearlin, linkine) and both the prismatic and the nacreous layers (shematrin 8, nacrein) following 2 months of exposure to 3 temperatures (21°C, 25°C and 28°C) and 2 microalgal concentrations 800 cell mL−1 for low food (LF) (light grey) and 15 000 cell mL−1 for high food (HF) (dark grey).
The results of two measures performed on a pool of 5 pearl oysters are shown for each condition. Statistical differences between temperatures are indicated by a letter.
Table 2.
Significance level of Scheirer-Ray–Hare (SHR) non-parametric ANOVA of calcifying genes expression level according to food and temperature levels.
Table 3.
Spearman’s coefficient correlation rho between relative matrix gene expression and SDR.
Table 4.
Regulation pattern of genes according to temperature (T), food (F) and shell deposition rate (SDR).