Figure 1.
Schematic drawing of the modified Y-tube olfactometer.
All dimensions are in millimeter.
Figure 2.
Preference Index (PI) of MDA, EA, BA, and DEET in both trials of experiment 1.
The response of non-blood fed Ae. aegypti females to MDA, EA, BA, and DEET did not change significantly between first and second trial (p = 0.440, 0.649, 0.313, and 0.170, respectively, paired t-test, n = 5).
Figure 3.
Preference Index (PI) of MDA, EA, BA, and DEET in experiment 1 and 2.
Non-blood fed Ae. aegypti females were repelled by MDA, EA, and DEET while BA did not have an effect on mosquito orientation. A) Pooled preference index from first and second trial in experiment 1 shows a repellent effect for MDA, EA, and DEET (p<0.001, Student t-test, n = 10) and no effect for BA (p = 0.922, Student t-test, n = 10). B) Preference index in experiment 2 shows a repellent effect for MDA, EA, and DEET (p = 0.004, 0.002, and <0.001, respectively, Student t-test, n = 7) and no effect for BA (p = 0.868, Student t-test, n = 7). Number of asterisks indicates the level of significance; p<0.05 (*), and p<0.001 (***).
Figure 4.
Oviposition Activity Index (OAI) of MDA, EA, BA, and DEET at three different concentrations.
MDA had no effect on oviposition at 1, 10, or 100(p = 0.396, 0.472, and 0.484, respectively, paired t-test, n = 5). There was no effect for EA at 1 ppm but deterred oviposition at 10 and 100 ppm (p = 0.774, 0.017, and <0.001, paired t-test, n = 5). BA had no effect at 1 ppm but strongly deterred oviposition at 10 and 100 ppm (p = 0.513, <0.001, and <0.001, respectively, paired t-test, n = 5). DEET had no effect at 1 and 10 ppm and a weak deterrent effect at 100 ppm (p = 0.487, 0.837, and 0.037, respectively, paired t-test, n = 5). Number of asterisks indicates the level of significance; p<0.05 (*), and p<0.001 (***).
Table 1.
Mean number of eggs laid on different concentrations of MDA, EA, BA, and DEET in comparison with water.