Table 1.
Primers used for detecting the target genes and the melting temperatures (Tm) used for primers.
Table 2.
Plasmids and PCR fragments used as standards.
Figure 1.
Raw gene copy numbers detected in a WWTP sample (copy number/ml).
A - 16S rRNA gene in inflow (IF) and effluent (EF). Assay1 was used only for samples from large WWTP (Helsinki) from Winter 2010 to Autumn 2011; B–Antibiotic resistance genes (ARGs). Statistical significance between inflow wastewater and effluent samples: *** - p<0.01; *0.03>p>0.01. For the pairs not marked the statistical difference between inflow and outflow was statistically insignificant. The line in each box marks the median and boxes: 25th and 75th percentiles; whiskers: 5th and 95th percentiles and outliers ±1.5 * IQR. See Figure S2 in File S1 for abundances of same genes presented by each sampling event.
Table 3.
Detection of ARGs in different WWTPs (total of all analyses per gene, n = 15).
Figure 2.
Antibiotic resistance gene copy numbers normalised to 16S rRNA gene copy numbers. The results are given for all samples for one gene for a WWTP, no seasonal comparison. Statistically significant comparison results are marked with *** at p<0.01, *0.03>p>0.01. The line in each box marks the median and boxes: 25th and 75th percentiles; whiskers: 5th and 95th percentiles and outliers ±1.5 * IQR.