Figure 1.
Experimental design for the study.
Table 1.
The macronutrient information of each diet.
Table 2.
The composition of the metabolic therapy used in this study.
Table 3.
Criteria of the neurological score.
Table 4.
The mean weight of organs and tissue samples collected from each group.
Figure 2.
Effect of treatment on body weight in SOD1-G93A mice.
There was no significant difference in body weight of SOD1-G93A mice between the different diet groups either before treatment started (Baseline), at presymptomatic stage (before neurological score 1) and at endpoint of the study. All data are mean ± SEM.
Figure 3.
Improved motor function was observed in KD and SD+DP groups.
A) KD group had significantly better result on accelerating rotarod during week 11 and 15 (p = 0.03, p = 0.018, respectively) when compared to the control group. (SD, n = 12; SD+DP, n = 11; KD, n = 9; KDDP, n = 9). B) Grip test showed better motor performance in KD group at week 15 (p = 0.015), while the motor function was significantly better at week 17 in SD+DP group (p = 0.039), compared to control. (SD, n = 13; SD+DP, n = 12; KD, n = 11; KDDP, n = 12). C) PaGE test showed better motor performance in KD group on week 15 and 16 (p = 0.027, p = 0.01), while the motor function was significantly better at week 12, 15, 16 and 17 in SD+DP group (p = 0.033, p = 0.023, p = 0.002, p = 0.019, respectively), when compared to SD group. All data are mean ± SEM. (SD, n = 9; SD+DP, n = 8; KD, n = 8; KDDP, n = 8). (*shows significant difference between SD and SD+DP groups; # shows significant difference between SD and KD+DP groups).
Figure 4.
SD+DP delayed disease progression in SOD1-G93 mice.
The difference in neurological score was significant between the control group (SD) and KD group on day 105 and 106, and the SD+DP group between day 118 and day 128 (except no data on day 119, 123, 124) implicating delayed disease progression in SD+DP group (p <0.05). All data are mean ± SEM.
Figure 5.
SD+DP increased survival time in SOD1-G93 mice.
Kaplan-Meier survival plot of study groups. Animals receiving SD+DP showed significantly longer survival compared to control animals (p = 0.001).
Table 5.
The mean age at endpoint, increase in lifespan and the number of animals survived past 125 days are shown for each group.