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Figure 1.

HRM method.

Examples of melting curves (A) and difference plots (B) obtained for wild-type (WT) and L367fs*46 and K385fs*47 mutations of CALR.

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Figure 2.

Limit of detection of HRM assay.

Melting curves (A) and difference plots (B) of wild-type (blue) and serial dilutions of a CALR K385fs*47 mutant (brown).

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Figure 2 Expand