Table 1.
Primer/Probe sequences and Amplicon length of target clock genes.
Figure 1.
Graphs illustrating the temporal organization (a–c), phase relationship (d, e) and relative amplitudes (f) of Per2, Bmal1 and Dbp mRNA rhythms in the SCN. The double plotted graphs show relative mRNA levels across 24 zeitgeber times fitted with a 24-h sine wave for Per2 (a), Bmal1 (b), and Dbp (c). Each point represents means ± SEM of mRNA data from 6–7 rats sampled every 30 min and grouped in consecutive 2-h intervals. R2 = Goodness of fit value for sine wave. n = 73 for all.
Figure 2.
Central amygdala, lateral part.
Graphs illustrating the temporal organization (a–c), phase relationship (d, e) and relative amplitudes (f) of Per2, Bmal1 and Dbp mRNA rhythms in the CEAl. The double plotted graphs show relative mRNA levels across 12 zeitgeber times fitted with a 24-h sine wave for Per2 (a), Bmal1 (b), and Dbp (c). Each point represents means ± SEM of mRNA data from 6–7 rats sampled every 30 min and grouped in consecutive 2-h intervals. R2 = Goodness of fit value for sine wave. n = 74 for all.
Figure 3.
Graphs illustrating the temporal organization (a–c), phase relationship (d, e) and relative amplitudes (f) of Per2, Bmal1 and Dbp mRNA rhythms in the DG. The double plotted graphs show relative mRNA levels across 12 zeitgeber times fitted with a 24-h sine wave for Per2 (a), Bmal1 (b), and Dbp (c). Each point represents means ± SEM of mRNA data from 6–7 rats sampled every 30 min and grouped in consecutive 2-h intervals. R2 = Goodness of fit value for sine wave. n = 74 for Bmal1 and Dbp, n = 73 for Per2.
Figure 4.
Temporal organization, phase relationship and amplitude of Per2, Bmal1 and Dbp expression in the SCN, CEAl and DG.
The double plotted line graphs show relative mRNA levels across 12 zeitgeber times fitted with a 24-h sine wave between the SCN, CEAl, and DG for Per2 (a), Bmal1 (b), and Dbp (c). The sine waves were fit to the data grouped in consecutive 2-h intervals as in figures 1–3. The phase relationship between the SCN, CEAl, and DG for each of the genes is depicted in the 24-h circular diagrams and the amplitude between these regions in the bar graphs. Asterisk indicates statistical significance (p<.05) as follows: *: CEAl compared to all other regions.
Figure 5.
Temporal organization and phase relationship of Per2 mRNA and PER2 in the SCN, CEAl and DG.
The double plotted line graphs show relative Per2 mRNA (red) and PER2 protein (blue) levels across 12 zeitgeber times fitted with a 24-h sine wave in the SCN (a), CEAl (b), and DG (c). The PER2 protein data have been re-plotted from a previously published report using the same rats used in the present study for comparison [3]. The sine waves were fit to the data grouped in consecutive 2-h intervals as in figures 1–3. The 24-h circular diagrams depict the phase relationship between Per2 mRNA and PER2 protein in each of the three regions analyzed.
Figure 6.
Peak clock gene expression in central and peripheral regions.
Twenty-four hour circular diagrams displaying peak Per2, Bmal1 and Dbp expression in the SCN, CEAl, DG, olfactory bulb (OB), liver and tail skin. Data for olfactory bulb, liver and skin are based on previously published results [8] using the same rats used in the present study for comparison.