Figure 1.
Rarefaction curves showing the relationship between the increase of bacterial α-diversity and the number of randomly sampled sequences.
A, number of OTUs observed experimentally; B, diversity predicted by Shannon index; C, richness predicted by Chao1; D, the sample coverage.
Table 1.
Overview of environmental data in hummock and hollow soils of the three wetlands (mean±SD, n = 3).
Figure 2.
Taxonomic assignment of sequences affiliated with Archaea from samples in different wetlands and microtopographies.
Error bars indicate the standard deviation of relative sequence abundance between three replicate samples.
Figure 3.
Relative sequence abundances of bacterial phyla and proteobacterial classes.
Major taxa detected with average relative sequence abundances >1% are displayed. Column “Bacteria-others” indicates combined relative sequence abundances of all the rare phyla, candidate divisions and of the taxonomically unclassified sequences; rare phyla were defined as having average relative sequence abundances between all samples of <1%.
Table 2.
Percent relative abundance of 16S rRNA genes in the pyrosequencing datasets associated with genera of methanogens and methanotrophs (mean±SD, n = 3).
Figure 4.
Heatmap showing the relative abundance of selected OTUs.
The samples and OTUs are clustered according to Manhattan distances. The colors correspond to the relative abundance of the OTUs in the samples, as indicated by the color legend. The taxonomy of each OTU is provided to the lowest-level attained during the classification. Abbreviations are used to indicate class (c), order (o), suborder (so), family (f) and genus (g). Two-way ANOVA for each OTU was performed using location (L), topography (T) and location and topography (L×T). P-values of the ANOVA are indicated as follows: * (P<0.05), ** (P<0.01), *** (P<0.001).
Figure 5.
Principal coordinate analysis (PcoA) plot of samples using the unweighted UniFrac distance metric.
Figure 6.
Canonical correspondence analysis (CCA) used to evaluate the effect of soil factors on the bacterial community structure.
Soil factors indicated in blue text include AP (available phosphorus), pH, SOC (soil organic carbon), TN (total nitrogen), T (mean annual air temperature), SM (soil moisture) and A (altitude).