Table 1.
PCR primers.
Figure 1.
Amino acid alignment of anuran MHC class I.
ClustalW alignment of MHC class I from Rhinella marina, Rana pipiens (MHC class I clone R6 Genbank accession no.: AF185587 and R9: AF185588) and Xenopus laevis (MHC class I haplotype g: AF188579; haplotype f: AF188580; haplotype r: AF188582 and haplotype j: AF188586); * indicate putative peptide binding residues as predicted from Flajnik et al. 1999.
Figure 2.
Phylogenetic tree comparing Rhinella marina MHC class I to other vertebrates.
Maximum likelihood phylogenetic tree using General Time Reversible model of nucleotide substitution with invariant sites (rooted on midpoint) of MHC class I in cane toad, Rhinella marina, African clawed frog, Xenopus laevis, northern leopard frog, Rana pipiens, Atlantic salmon, Salmo salar, whitespotted bamboo shark, Chiloscyllium plagiosum, nurse shark, Ginglymostoma cirratum, chicken, Gallus gallus, platypus, Ornithorhynchus anatinus, gray short-tailed opossum, Monodelphis domestica, red-necked wallaby, Macropus rufogriseus, common chimpanzee, Pan troglodytes, and human, Homo sapiens. Sequence titles include Genbank accessions.
Figure 3.
Amino acid alignment of MHC class I alpha 1 domain and alpha 2 domain variants.
Putative peptide binding sites are indicated with * under each alignment.
Figure 4.
Phylogenetic trees for MHC class I alpha 1 domain and alpha 2 domain.
Maximum likelihood phylogenetic trees using General Time Reversible model of nucleotide substitution with invariant sites (rooted on midpoint) comparing Rhinella marina (Rhma) to other anuran species (Xenopus laevis, Xela; Silurana tropicalis, Sitr; Rana temporaria, Rate; Agalychnis callidryas, Agca; Espadarana prosoblepon, Espr; Smilisca phaeota, Smph; Lithobates catesbeianus, Lica; L. clamitans, Licl; L. yavapaiensis, Liya) and axolotl (Ambystoma mexicanium, Amme). Sequence titles include Genbank accessions.
Table 2.
Numbers of allele variants sequenced from cDNA from various tissues and the genomic DNA of one cane toad individual from Normanton, Australia.
Table 3.
Comparison of allelic diversity at the MHC class I (N is number of individuals studies and NA is number of alleles).