Figure 1.
Portable system for ion channel current recordings.
Droplet contact method for reproducible and stable lipid bilayer formation, (a). Illustration of the double-well (DW) chip used for ion channel measurement with the droplet contact method, (b). Schematic diagram of the channel current recordings for alpha-hemolysin reconstituted in the lipid bilayer, (c). Photograph of our portable system containing the DW chip with a handheld amplifier connected to a laptop PC, (d).
Figure 2.
Frequency-dependent power density and diagrams of two different electrode systems: inserted and bottom-well, (a).
Time required for αHL reconstitution using the drop-off (b) and repainting (c) methods, (b,c).
Figure 3.
Photograph of the recording system, DW chip with an amplifier connected to a PC, at a high-altitude site (near the summit of Mount Fuji), (a).
Current time frequency histogram of actual data taken at the field site, (b). Power density spectra of the open channel current of αHL in the field and the laboratory, (c).