Figure 1.
Normal (tolerance in healthy controls) and pathological (broken tolerance in RA) BCR signaling in autoreactive B cells with induced peripheral immune tolerance.
Figure 2.
BCR signaling pathways associated with the loss of peripheral induced tolerance in autoreactive BND cells of RA patients [2]. (A)
Unmanipulated BND cells: increased baseline activity Blnk, SHP and Jnk. (B) Response to BCR engagement in BND cells: decreased phosphorylation of Blnk, Syk, SHP2, CD19 and increased activation of Erk1/2, Jnk.
Figure 3.
Phosphorylation levels of major BCR signal transduction proteins and total tyrosine phosphorylation levels (pTyr) in CD27−IgD+IgMlow/− B cells of RA patients (red) and healthy control subjects (blue) at baseline and in response to anti-BCR stimulation in vitro.
Figure 4.
Induced tolerance status index (ITSI) discriminates between autoreactive BND cells from healthy controls and RA subjects based on BCR phosphoprotein activation patterns.
Table 1.
Strategies for BCR phosphoprotein data analysis in autoreactive BND cells.